| 抗烟曲霉单克隆抗体用于侵袭性烟曲霉感染病原学检测的实验研究 |
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| 引用本文： | 钟晓祝,郝卫,潘玉先,余力,车小燕,封志纯.抗烟曲霉单克隆抗体用于侵袭性烟曲霉感染病原学检测的实验研究[J].中华医院感染学杂志,2006,16(12):1340-1342. |
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| 作者姓名： | 钟晓祝 郝卫 潘玉先 余力 车小燕 封志纯 |
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| 作者单位： | 南方医科大学珠江医院,广东,广州,510282 |
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| 摘 要： | 目的探讨自行研制的抗烟曲霉单克隆抗体在兔侵袭性烟曲霉感染动物模型中的应用。方法建立兔侵袭性烟曲霉感染动物模型;用双抗体夹心酶联免疫吸附试验法(ELISA)检测兔血清中烟曲霉抗原;以免疫酶组织化学方法检测兔肺、肝、脾、肾组织中烟曲霉抗原。结果血清中烟曲霉抗原检测:接种烟曲霉抗原后24、48、72、96 h,血清中烟曲霉抗原检测阳性;免疫酶组织化学检测:接种后96 h,肺、肝、脾、肾组织免疫酶组织化学染色均为阳性。结论抗烟曲霉单克隆抗体在侵袭性烟曲霉感染的病原学诊断中有较大的应用前景。
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| 关 键 词： | 烟曲霉菌 单克隆抗体 感染 免疫酶组织化学 |
| 文章编号： | 1005-4529（2006）12-1340-03 |
| 收稿时间： | 2006-03-12 |
| 修稿时间： | 2006-05-20 |
Etiological Diagnosis of Invasive Aspergillosis by Monoclonal Antibodies Against Aspergillus fumigatus |
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| ZHONG Xiao-zhu,HAO Wei,PAN Yu-xian,YU Li,CHE Xiao-yan,FENG Zhi-chun.Etiological Diagnosis of Invasive Aspergillosis by Monoclonal Antibodies Against Aspergillus fumigatus[J].Chinese Journal of Nosocomiology,2006,16(12):1340-1342. |
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| Authors: | ZHONG Xiao-zhu HAO Wei PAN Yu-xian YU Li CHE Xiao-yan FENG Zhi-chun |
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| Institution: | Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China |
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| Abstract: | OBJECTIVE To study the etiological diagnosis of invasive aspergillosis by the monoclonal antibodies against Aspergillus fumigatus. METHODS An animal model of rabbit invasive aspergillosis was established.The antigen of A.fumigatus in serum was detected by ELISA.The antigen of A.fumigatus in tissue was detected by immunochemistry. RESULTS ELISA assay showed positive 24,48 and 72 hours after infection.Immunochemistry was positive 72 hours after infection. CONCLUSIONS The monoclonal antibodies against A.fumigatus has great potency usage. |
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| Keywords: | ELISA |
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