| PDGF-AB对成纤维细胞增殖及DNA合成的影响 |
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| 引用本文： | 董茂龙,陈璧,朱雄翔,李永林,汤朝武. PDGF-AB对成纤维细胞增殖及DNA合成的影响[J]. 西北国防医学杂志, 1999, 20(4): 274-276 |
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| 作者姓名： | 董茂龙 陈璧 朱雄翔 李永林 汤朝武 |
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| 作者单位： | 第四军医大学西京医院烧伤外科,西安,710033 |
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| 摘 要： | 目的：探讨ＰＤＧＦ－ ＡＢ促进伤口愈合及其在瘢痕增生中的作用机理。方法：取体外培养的人正常皮肤及增生性瘢痕成纤维细胞（ｎｏｒｍａｌｓｋｉｎｆｉｂｒｏｂｌａｓｔ，ＮｓＦｂ，ｈｙｐｅｒｔｒｏｐｈｉｃ ｓｃａｒｆｉｂｒｏｂｌａｓｔ，ＨＴｓＦｂ） ，用ＭＴＴ和３Ｈ－ ＴｄＲ掺入法观察ＰＤＧＦ－ ＡＢ对两种细胞增殖及ＤＮＡ合成的影响。结果：ＰＤＧＦ－ ＡＢ对两种成纤维细胞增殖及ＤＮＡ合成均有明显刺激作用，且均呈剂量依赖性关系，但作用有差异。结论：ＰＤＧＦ－ ＡＢ可能通过刺激成纤维细胞增殖及ＤＮＡ合成促进伤口愈合，但同时在瘢痕增生性疾病中可能起重要促进作用。
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| 关 键 词： | PDGF-AB MTT实验 DNA合成 ~3H-TdR掺入 |
The effect of PDGF-AB on proliferation and DNA synthesis of fibroblasts |
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| DONG Mao long,CHEN Bi,ZHU Xiong xiang,et al.. The effect of PDGF-AB on proliferation and DNA synthesis of fibroblasts[J]. Medical Journal of National Defending Forces in Northwest China, 1999, 20(4): 274-276 |
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| Authors: | DONG Mao long CHEN Bi ZHU Xiong xiang et al. |
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| Affiliation: | DONG Mao long,CHEN Bi,ZHU Xiong xiang,et al . |
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| Abstract: | Purpose To investigate the mechanism by which PDGF-AB promotes wound healing and the effect of PDGF-AB on scar forming.Methods:In vitro cultured human normal skin and hypertrophic scar fibroblasts were applied to measure the effect of PDGF-AB on proliferation and DNA synthesis of fibroblasts by MTT and 3 H-TdR incorporation.Results:There was difference of proliferation and DNA synthesis between human normal skin and hypertrophic scar fibroblasts,PDGF-AB could enhance proliferation and DNA synthesis of two kinds of fibroblasts in dependent dose.Conclusion:PDGF-AB may promote wound healing by stimulating proliferation and DNA synthesis of fibroblasts,and may play important role in scar forming. |
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| Keywords: | PDGF-AB MTT test DNA synthesis 3H-TdR incorporation |
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