氯化锂对大鼠皮层CCK阳性神经元数的影响

目的观察慢性给锂大鼠皮层胆囊收缩素(CCK)阳性神经元数及体重变化,阐明长期锂作用对大鼠脑发育及学习记忆能力的影响。方法将大鼠随机分为对照组和4个氯化锂(LiCl)组,分别给予普通饲料和含LiCl(3,30,300,3000mg/kg)的饲料喂养60d,通过测量体重和Y-迷宫实验,采用卵白素-生物素-酶复合物(ABC)免疫组化法观测大鼠皮层CCK阳性神经元的变化。结果3,30mg/kgLiCl组大鼠体重增量和Y-迷宫实验成绩均明显好于正常对照组(P<0.01),300,3000mg/kgLiCl组大鼠则明显差于对照组(P<0.05)。3,30,300mg/kgLiCl组大鼠皮层CCK阳性神经元数较对照组增多,3000mg/kgLiCl组则明显减少(P<0.05)。结论较低浓度的锂能促进大鼠体格发育,提高学习记忆能力,增强皮层CCK蛋白表达;而高浓度锂会产生负面影响。

Effects of lithium on cortical cholecystokinin positive neurons in rats

Objective To investigate the effects of lithium on brain development,learning ability and memory capacity of rats.Methods Wistar rats were randomly divided into control group and four lithium chlorde groups fed with normal food and different dosage LiCl(3,30,300and300mg/kg) respectively.By means of measuring the body weight,using Y-maze test and ABC immunohistochemistry.The different of the body weight gains and Y-maze training times of different groups and the changes of CCK positive neurons in cortical were observed.Results Compared with the cnotrol group,3,30mg/kg LiCl groups could increase the body weight and improve the ability of learning and memory of rats (P<0.01),however,those of the rats of 300,3000mg/kg LiCl group were lower (P<0.05).The number of CCK positive neurons in cortical of 3,30,300mg/kg LiCl groups were significantly increased compared with the control group (P<0.05).The number of CCK positive neurons of 3000mg/kg LiCl group was lower than that of the control group (P>0.05).Conclusion Low dose of lithium could improve the growth;the learning ability and the memory capacity obviously ,but high dose of lithium could result in harmful effects.