脑源性神经营养因子融合蛋白对大鼠局灶性脑缺血治疗作用的初步研究

目的 研究脑源性神经营养因子融合蛋白(BDNF-PTD)在大鼠脑缺血模型中对大脑神经元的保护作用.方法 SD大鼠采用随机数字表法分为给药模型组、空白模型组、阴性对照组、正常组,每组5只.前3组大鼠通过线栓法制作脑中动脉栓塞模型;正常组大鼠正常喂养.不做任何处理.栓塞1 h后给药模型组腹腔注射50 μg(50 μg/mL)BDNF-PTD,阴性对照组注射1 mL生理盐水,空白模型组不做处理.采用Bederson评分评价各组大鼠神经功能缺损情况.24h后将各组大鼠断头取脑,大脑切片TTC染色,观察各组大鼠脑梗死面积.结果 给药模型组大鼠神经功能缺损不明显,Bederson评分明显低于空白模型组及阴性对照组(P<0.05).TTC染色结果提示给药模型组大鼠脑梗死区域面积较空白模型组、阴性对照组明显缩小,差异有统计学意义(P<0.05).结论 BDNF-PTD能通过血脑屏障并且发挥其生物学效应.起到保护脑缺血后神经元作用.

Effects of BDNF-PTD fusion protein on treatment of focal cerebral ischemia in SD rats

Objective To investigate the neuroprotective effects of BDNF-PTD fusion protein on rats with focal cerebral ischcmia. Methods We divided the SD rats into BDNF-PTD fusion protein-treated group,blank model group,negative control group and normal group(n=5).Middle cerebral arterial occlusion(MCAO)models were established by suture methods.BDNF-PTD was intraperitoneally injected into the BDNF-PTD fusion protein-treated group at a dosage of 50 μg(50 μg/mL)and negative control group was injected 1 mL saline 1 h after the success of model making.The deficit of nerve function was eveluated by Bederson score.Brain slices were stained by TTC and the area of infaretion was measured and analyzed by image J software. Results Neurologic impairment in the BDNF-PTD fusion protein-treated group was not statistically obvious(P>0.05);while its Bederson scores was significantly lower as compared with those in the blank model group and negative control group (P<0.05).TTC staining showed that the ischemic area in the BDNF-PTD fusion protein-treated group was significantly decreased as compared with that in the blank model group and negative control group (P<0.05). Conclusion BDNF-PTD fusion protein can play a neuroprotective effect on the neuron after cerebral ischemia through the blood-brain barrier and its biological effects.