复肝春6号对小鼠肝癌的抑制作用及对荷瘤小鼠免疫功能的影响

目的：研究复肝春6号（FGC-6）的抗肝癌作用及对荷瘤小鼠免疫功能的影响。方法：将接种H22肝癌细胞的小鼠分为模型组、5-氟尿嘧啶组（5-Fu组）、FGC-6 高、中、低剂量组（含生药量70,35,15 g·kg^-1）,另设正常对照组,连续给药10 d。观察小鼠瘤重、体重、NK细胞杀靶活性、淋巴细胞增殖活性及脾细胞白细胞介素2（IL-2）分泌量的变化。制备FGC-6含药血清,以MTT法和生长曲线法观察该含药血清对体外培养H22细胞的增殖抑制作用。 结果：FGC-6高剂量可明显抑制荷瘤小鼠移植瘤的生长,且FGC-6含药血清对H22细胞增殖有明显的抑制作用,呈时间和浓度依赖性。模型组荷瘤小鼠的NK细胞杀靶活性和脾细胞IL-2分泌量均明显低于正常对照组（P<0.05）； 而FGC-6高剂量组荷瘤小鼠的NK细胞杀靶活性、脾细胞IL-2分泌量和淋巴细胞增殖活性均得到明显改善,FGC-6中剂量治疗使荷瘤小鼠的脾细胞IL-2分泌能力增强,与模型组比较差异显著（P<0.05）。 结论： FGC-6可明显抑制H22细胞的生长,改善荷瘤小鼠的免疫功能。

Study on effects of anticancer and immunoregulation of Fuganchun 6 on hepatoma of mouse

Objective: To study the anticancerous effect of Fuganchun 6(FGC-6) and its immunoregulatory effect on tumor-bearing mice.Method: The mice inoculated by H22 cells were divided into 5 groups: model group,5-Fu group and FGC-6 in high dose,medium dose,and low dose groups.The normal mice were also observed.These mice were treated for 10 days.The weight of tumor mass and mouse were examined.The target-cell-killing activity of NK cells.The proliferation activity of lymphocyte and the production of IL-2 of murine splenocytes were detected respectively.The serum containing FGC-6 was prepared and its inhibition effect on H22 cells was examined by MTT assay and growth curve in vitro.Result: Growth of tumor was inhibited markedly by FGC-6 high dose.The inhibition of serum containing FGC-6 on the proliferation of H22 cells in vitro was observerd in a dose and timedependent manner.The target-cell-killing activity of NK cells and the production of IL-2 of murine splenocytes of model group were lower than those of normal group(P<0.05).When compared with model group,FGC-6 in high dose elevated the two indexes above-mentioned,and also enhanced the proliferation activity of lymphocyte markedly(P<0.05).The production of IL-2 of murine splenocytes was also improved when treated by FGC-6 in medium dose(P<0.05).Conclusion: FGC-6 can inhibite the growth of H22 cells markedly and also can strengthen the immunity of H22 transplanted mouse.