尿白介素—8检测在狼疮性肾炎病情判断中的价值

目的探讨尿白介素 - 8(IL- 8)检测在狼疮性肾炎 (L N)病情判断中的价值。方法应用 EL ISA双抗体夹心法检测 38例 L N患者尿 IL - 8水平。结果 1L N患者肾功正常组和肾功不全代偿期组尿 IL - 8水平显著高于健康人 (P<0 .0 0 1) ,且肾功不全代偿期组尿 IL- 8水平显著高于肾功能正常组 (P<0 .0 1) ,高尿 IL- 8水平患者伴较高的血沉值和较低的补体 C3值。2出现白细胞尿的 L N患者尿 IL- 8水平显著高于其它患者 (P<0 .0 5 )。3环磷酰胺静脉冲击加强的松治疗 4周后 ,L N患者尿 IL- 8水平显著降低 (P<0 .0 1)。结论尿 IL - 8水平的变化可能有助于判断狼疮活动和肾损害程度。L N患者非感染性白细胞尿的出现可能与尿 IL- 8水平增高有关。环磷酰胺和强的松可能通过抑制免疫细胞和肾固有细胞产生 IL- 8而取得疗效

Clinical significance of urinary IL-8 in patients with lupus nephritis

ObjectiveTo evaluate the clinical value of detection of urinary interleukin 8(IL 8) in patients with lupus nephritis (LN) MethodsUrinary levels of IL 8 were determined by using sandwich ELISA in 38 LN patients ResultsIn the LN patients during the stage of normal renal function and diminished renal reserve stage, urinary levels of IL 8 were significantly higher than those in healthy controls( P <0 001),and urinary levels of IL 8 in the patients during diminished renal reserve stage were significantly higher than those in the patients during normal renal function stage( P <0 01) High IL 8 levels in LN patients were associated with high levels of erythrocyte sedimentation rate and lower levels of C3 complement components Urinary levels of IL 8 in LN patients with microscopic leukocyturia were significantly higher than those without microscopic leukocyturia In LN patients treated with cyclophosphamide (CTX) and prednisone, urinary IL 8 levels of post treatment were significantly lower than those of pre treatment( P <0 01) Conclusions Urinary levels of IL 8 may be a useful indicator for monitoring disease activity and degree of renal damage in LN The mechanism of noninfective leukocyturia may be associated with the elevation of urinary IL 8 levels in LN CTX and prednisone may have a significant effect on inhibiting the production of IL 8 in the immunocytes and renal intrinsic cells