白血病抑制因子抑制神经元凋亡及促进内源性神经干细胞的增殖

背景:研究表明白血病抑制因子能提升内源性神经干细胞的增殖及改变其分化方向,但还没有研究证实其是否能影响神经元的凋亡。目的:观察白血病抑制因子干预下神经元的凋亡及内源性神经干细胞的增殖情况,以及二者是否有一定相关性。方法:c57BL小鼠32只,随机分为假手术组,白血病抑制因子组,帕金森病组及正常对照组。除正常对照组外,其他3组均接受三维脑立体定向注射6-羟基多巴胺制备小鼠帕金森病模型。造模后2h,假手术组只接受ALZET锇药物泵导管植入术,但不植入泵导管;白血病抑制因子组接受ALZET锇药物泵导管植入术,经泵导管将白血病抑制因子直接缓慢释放到脑脊液中,帕金森病组给予生理盐水。结果与结论:与帕金森病组相比,白血病抑制因子组小鼠运动功能明显改善,内源性神经干细胞显著增殖,凋亡细胞数呈显著性下降,二者之间存在相互关系。白血病抑制因子可能是一种能有效重建变性神经系统的神经营养因子。

Leukemia inhibitory factor decreases the apoptosis of neurons and promotes the proliferation of endogenous neural stem cells

BACKGROUND: Studies have shown that leukemia inhibitory factor (LIF) can enhance the proliferation of endogenous neural stem cells (ENSCs), and change their differentiation direction, but no studies have domenstrated whether LIF can affect neuronal apoptosis. OBJECTIVE: To detect the neuron apoptosis and the proliferation of ENSCs in mouse models after LIF administration. METHODS: Thirty-two c57BL mice were randomized into sham-operation group, LIF group, Parkinson’s disease (PD) group and control group. Except the control group, mice in the other groups were injected with 6-hydroxydopamine to make PD models. LIF group received ALZET osmium pump duct implantation, and infused with normal saline containing LIF (25 μg/kg) into the cerebrospinal fluid. PD group received ALZET osmium pump duct implantation, and only infused with normal saline into the cerebrospinal fluid. Sham-operation group received ALZET osmium pump duct implantation without the pump duct. RESULTS AND CONCLUSION: LIF group showed a statistically significant improvement in motor functioin, compared with PD group; the number of ENSCs increased and the number of apoptosis neurons decreased significantly in the LIF group compared with PD group. It is possible that LIF is a neurotrophic factor for effectively rebuilding the degenerated central nervous system.