| 血红素氧合酶1提高骨髓间充质干细胞在梗死后心脏的存活 |
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| 引用本文： | 曾彬,易欣,马乐乐. 血红素氧合酶1提高骨髓间充质干细胞在梗死后心脏的存活[J]. 中国组织工程研究与临床康复, 2011, 15(40). DOI: 10.3969/j.issn.1673-8225.2011.40.002 |
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| 作者姓名： | 曾彬 易欣 马乐乐 |
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| 作者单位： | 武汉大学人民医院心血管内科,湖北省武汉市,430060 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 背景:移植骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)修复梗死后心功能受到围移植期移植细胞大量死亡的限制.因此寻找一种保护因子对移植细胞提供保护至关重要.目的:观察血红素氧合酶1(heme oxygenase,HO-1)对BMSCs在梗死后心脏生存的影响.方法:体外分离扩增培养大鼠BMSCs,Adv-hHO-1、Adv-GFP分别转染,移植前DAPI标记.结扎左前降支1 h后,分别将DAPI-hHO-1-BMSCs、DAPI-GFP-BMSCs多点注射到大鼠心脏梗死区周边,对照组注射等量PBS.结果与结论:Adv-hHO-1转染BMSCs后获稳定表达.仅hHO-1-BMSCs组稳定表达hHO-1 mRNA;hHO-1-BMSCs组血管内皮生长因子、碱性成纤维细胞生长因子、肝细胞生长因子表达均高于其他两组(P < 0.01);存活BMSCs数量在第3,7天均明显高于GFP-BMSCs组(P < 0.05);大鼠心功能各项参数明显优于其他两组(P < 0.01).移植4周后,HO-1-BMSCs组梗死区周边毛细血管密度明显高于GFP-BMSCs组和对照组(P < 0.01),且胶原蛋白沉积减少,心室壁变厚,梗死面积较其他两组明显缩小(P < 0.01).提示HO-1提高移植到梗死心脏BMSCs的存活,HO-1协同BMSCs抑制心室重构,改善心功能.
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| 关 键 词： | 血红素氧合酶1 骨髓间充质干细胞 心肌梗死 细胞因子 血管新生 |
Human heme oxygenase-1 improves the survival of bone marrow mesenchymal stem cells in ischemic heart |
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| Zeng Bin,Yi Xin,Ma Le-le. Human heme oxygenase-1 improves the survival of bone marrow mesenchymal stem cells in ischemic heart[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(40). DOI: 10.3969/j.issn.1673-8225.2011.40.002 |
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| Authors: | Zeng Bin Yi Xin Ma Le-le |
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| Affiliation: | Zeng Bin,Yi Xin,Ma Le-le Department of Cardiology,Renmin Hospital of Wuhan University,Wuhan 430060,Hubei Province,China |
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| Abstract: | BACKGROUND: Cardiac function is limited by the great amount of dead transplanted cells following myocardial infarction (AMI) treatment with bone marrow mesenchymal stem cells (BMSCs) transplantation. OBJECTIVE: To investigate the effects of human heme oxygenase-1 (HO-1) on the survival of BMSCs in the heart after AMI. METHODS: BMSCs were isolated, cultured and proliferated in vitro, transfected with Adv-hHO-1 and Adv-GFP, and then labeled with DAPI before transplantation. At 1 hour after left coronary arter... |
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