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| 多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜的制备及降解性能 | |
| 引用本文： | 吴岳恒,张灵敏,曾祥君,麦丽萍,武长礼,汤顺清,余细勇.多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜的制备及降解性能[J].中国组织工程研究与临床康复,2011,15(51):9531-9534. |
| 作者姓名： | 吴岳恒 张灵敏 曾祥君 麦丽萍 武长礼 汤顺清 余细勇 |
| 作者单位： | 1. 广东省医学科学院,广东省人民医院医学研究中心,广东省广州市,510080 2. 暨南大学生物医学工程所,广东省广州市,510632 3. 广东省医学科学院,广东省心血管病研究所心外科,广东省广州市,510080 |
| 基金项目： | 国家自然科学基金资助项目(30901468),课题名称:偏磷酸钙微粒改善多孔聚四羟丁酸膜细胞亲和性的实验研究~~ |
| 摘 要： | 背景:种子细胞能否成功黏附于支架材料表面,与支架材料的降解性能密切相关。目的:制备多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜,并观察其体外降解性能。方法:将聚3-羟基丁酸-4-羟基丁酸和偏磷酸钙按50∶50混合均匀,完全溶解于氯仿后,搅拌均匀,倾倒在培养皿上,干燥后即成多孔膜。将其置于循环装置中,流速为0.30m/s,管路内灌满37℃生理盐水50mL。结果与结论:流场作用多孔复合膜2,4,8,16,24h后,其失重率分别为4.5%,5.6%,6.9%,9.6%和10.6%;扫描电镜显示,降解24h后,表面偏磷酸钙微粒已降解完全或脱落,而内凹偏磷酸钙微粒仍嵌入在聚3-羟基丁酸-4-羟基丁酸复合膜内,复合膜表面直径2μm的微孔基本不见,直径30~50μm的大孔基本未变,但孔隙边缘变得圆滑;水静力称重法计算出降解实验前后复合膜吸水率、显气孔率和密度分别为降解前的40.6%,46.3%,1.14g/cm3和降解后的51.6%,54.1%1.05g/cm3。说明实验制备的多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜降解早期以偏磷酸钙降解为主,降解过程中孔内微丝易降解或断裂。 |
| 关 键 词： | 聚3-羟基丁酸-4-羟基丁酸 降解 偏磷酸钙 复合膜 材料 吸水率 显气孔率 密度 |
Preparation and degradation property of composite membrane of porous poly (3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate |
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| Wu Yue-heng,Zhang Ling-min,Zeng Xiang-jun,Mai Li-ping,Wu Chang-li,Tang Shun-qing,Yu Xi-yong.Preparation and degradation property of composite membrane of porous poly (3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(51):9531-9534. | |
| Authors: | Wu Yue-heng Zhang Ling-min Zeng Xiang-jun Mai Li-ping Wu Chang-li Tang Shun-qing Yu Xi-yong |
| Institution: | Wu Yue-heng1,Zhang Ling-min2,Zeng Xiang-jun3,Mai Li-ping1,Wu Chang-li3,Tang Shun-qing2,Yu Xi-yong1 1Center of Medical Research,Guangdong General Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510080,Guangdong Province,China,2Institute of Biomedical Engineering,Jinan University,Guangzhou 510632,3Department of Cardiac Surgery,Guangdong Cardiovascular Institute |
| Abstract: | BACKGROUND: Degradation properties of scaffold are closely related to the adhering of seed cells to its surface. OBJECTIVE: To prepare the composite membrane of porous poly (3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate (P (3HB-co-4HB)/CMP) and observe its degradation properties in vitro. METHODS: The raw material ratio of P (3HB-co-4HB)/CMP was 50/50, and the P (3HB-co-4HB)/CMP was mixed uniformly and dissolved in chloroform and dumped in a Petri dish. After drying, the porous composite mem... |
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