| 昆明小鼠3.5d和4d囊胚不同分离方法的比较 |
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| 引用本文： | 邵晓云,徐绍业. 昆明小鼠3.5d和4d囊胚不同分离方法的比较[J]. 中国组织工程研究与临床康复, 2011, 15(40). DOI: 10.3969/j.issn.1673-8225.2011.40.028 |
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| 作者姓名： | 邵晓云 徐绍业 |
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| 作者单位： | 1. 桂林医学院,组织学与胚胎学教研室,广西壮族自治区桂林市541004
2. 桂林医学院,科学实验中心,广西壮族自治区桂林市541004 |
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| 基金项目： | 基础医学院科研启动基金 |
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| 摘 要： | 背景:远交系的昆明小鼠作为中国自然科学研究主要实验动物,其胚胎干细胞建系的成功率一直很低.目的:探讨昆明小鼠胚胎干细胞体外分离培养的最佳方法和最适合的采胚时间.方法:采集孕3.5 d和4 d的囊胚分别以免疫外科法和全胚培养法在小鼠胚胎成纤维细胞饲养层上分离和克隆昆明小鼠胚胎干细胞集落.结果与结论:免疫外科法和全胚法培养3.5 d囊胚的内细胞团贴壁率和原代克隆形成率差异均不显著(P > 0.05);全胚法培养4 d囊胚的内细胞团贴壁率显著高于免疫外科法(P < 0.05),但原代克隆形成率显著低于免疫外科法(P < 0.05);全胚法培养4 d囊胚的原代克隆形成率显著高于3.5 d囊胚(P < 0.05);免疫外科法分离4 d囊胚内细胞团的贴壁率和原代克隆形成率显著高于以同样方法分离培养的3.5 d囊胚(P < 0.05).结果显示用免疫外科法分离4 d囊胚更适合于昆明小鼠胚胎干细胞的体外分离培养.
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| 关 键 词： | 昆明小鼠 胚胎干细胞 全胚法 免疫外科 内细胞团 |
Comparison of different methods to isolate 3.5-day and 4-day blastocysts from Kunming mice |
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| Shao Xiao-yun,Xu Shao-ye. Comparison of different methods to isolate 3.5-day and 4-day blastocysts from Kunming mice[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(40). DOI: 10.3969/j.issn.1673-8225.2011.40.028 |
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| Authors: | Shao Xiao-yun Xu Shao-ye |
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| Affiliation: | Shao Xiao-yun1,Xu Shao-ye2 1Department of Histology and Embryology,2Science and Technology Division,Guilin Medical University,Guilin 541004,Guangxi Zhuang Autonomous Region,China |
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| Abstract: | BACKGROUND: Outbreed Kunming mice as the main experimental animals for natural science research in China have a low successful rate in establishment of embryonic stem cell line. OBJECTIVE: To explore the method of isolation and culture of murine embryonic stem cells and the best time to collect embryos of Kunming species. METHODS: Blastocysts obtained from 3.5-day and 4-day embryos after mating were treated with intact embryo cultivation and immuosurgery. Inner cell masses were isolated and cultured on a fe... |
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