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| 结直肠癌组织中CD44+肿瘤细胞与S期标志物增殖细胞核抗原的关系 | |
| 引用本文： | 王芙蓉,刘斌,苏勤军,杨艳丽,钱震,史敏. 结直肠癌组织中CD44+肿瘤细胞与S期标志物增殖细胞核抗原的关系[J]. 中国组织工程研究与临床康复, 2011, 15(40). DOI: 10.3969/j.issn.1673-8225.2011.40.029 |
| 作者姓名： | 王芙蓉 刘斌 苏勤军 杨艳丽 钱震 史敏 |
| 作者单位： | 1. 解放军兰州军区兰州总医院病理科,甘肃省兰州市,730050;兰州大学病理研究所,甘肃省兰州市730000 2. 解放军兰州军区兰州总医院病理科,甘肃省兰州市,730050;兰州大学病理研究所,甘肃省兰州市730000;甘肃省干细胞与基因药物重点实验室,甘肃省兰州市730050 3. 解放军兰州军区兰州总医院病理科,甘肃省兰州市,730050 |
| 基金项目： | 甘肃省自然科学基金资助项目 |
| 摘 要： | 背景:目前尚未找到结直肠癌干细胞理想的标志物,结合周期蛋白能更精确的找到GO/G1期癌干细胞.目的:了解结直肠癌组织中CD44和增殖细胞核抗原PCNA的表达及CD44+肿瘤细胞与S期标志物增殖细胞核抗原PCNA的关系.方法:采用组织芯片检测、免疫组织化学和双重免疫组织化学染色技术检测61例结直肠癌组织、10例正常肠黏膜组织中增殖细胞核抗原PCNA和CD44表达情况.结果与结论:在结直肠癌组织中,PCNA+细胞数远多于CD44+细胞数,CD44+癌细胞形态有两种,一种核较大,染色质边集,呈空泡状,核膜核仁清晰,这些细胞大多数呈PCNA阳性;另一种核较小,染色质致密,呈小圆形,部分表达PCNA.在大多数病例中,CD44+细胞中90%以上的瘤细胞同时为PCNA阳性,只有(3.38±2.08)%细胞为单纯PCNA阳性.说明结直肠癌组织中大多数CD44+细胞呈现S期标志物PCNA阳性,处于S期;只有极少数CD44+/PCNA-处于G0/G1期的细胞可能为肿瘤干细胞. |
| 关 键 词： | 肿瘤干细胞 结直肠癌 CD44 PCNA G0/G1 细胞周期 组织芯片 |
Relationship of CD44+ tumor cells and S-phase marker proliferating cell nuclear antigen in colorectal carcinoma |
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| Wang Fu-rong,Liu Bin,Su Qin-jun,Yang Yan-li,Qian Zhen,Shi Min. Relationship of CD44+ tumor cells and S-phase marker proliferating cell nuclear antigen in colorectal carcinoma[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(40). DOI: 10.3969/j.issn.1673-8225.2011.40.029 | |
| Authors: | Wang Fu-rong Liu Bin Su Qin-jun Yang Yan-li Qian Zhen Shi Min |
| Affiliation: | Wang Fu-rong1,2,Liu Bin1,3,Su Qin-jun1,Yang Yan-li1,Qian Zhen1,Shi Min1 1Department of Pathology,Lanzhou General Hospital of Lanzhou Military Command of Chinese PLA,Lanzhou 730050,Gansu Province,China,2Institute of Pathology,Lanzhou University,Lanzhou 730000,3Key Laboratory of Stem Cells and Gene Medicine |
| Abstract: | BACKGROUND: An ideal surface marker of cancer stem cells has not been found in colorectal carcinoma and it is very useful to find cancer stem cells in G0/G1 phase accurately by detecting the cell cycle. OBJECTIVE: To investigate the expression of CD44 and proliferating cell nuclear antigen (PCNA) and the relationship between CD44+ tumor cells and S-phase marker PCNA in colorectal carcinoma. METHODS: The expression of CD44 and PCNA were detected by tissue chip, immunohistochemical staining and double immunoh... |
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