镍钛形状记忆合金表面改性后的血液相容性

背景:镍钛形状记忆合金植入人体内必需要有很好的生物相容性和生物安全性。目的:观察分析镍钛形状记忆合金表面改性后对其生物相容性的影响。方法:将镍钛形状记忆合金随机分为两组,经阴极电沉积法处理的材料为实验组,未经处理的为空白组,通过扫描电镜观察实验组材料表面的变化,并测定材料的溶血率和动态凝血时间。体外培养骨髓基质干细胞,与两组材料复合培养,通过MTT法检测两组材料中细胞存活数量。结果与结论:镍钛形状记忆合金表面改性后,表面出现由很多的纳米级颗粒紧密聚集形成的Ti-O膜,实验组材料的溶血率下降,凝血时间延长,两组材料与骨髓基质干细胞复合培养第2,4,6天,实验组吸光度比空白组明显增高(P<0.05)。说明镍钛形状记忆合金经阴极电沉积法表面改性后具有较好的生物相容性和生物安全性。

Effect of surface treatment on the hemocompatibility of Ni-Ti shape memory alloy

BACKGROUND: Implantable Ni-Ti shape memory alloys must have better biocompatibility and biological security. OBJECTIVE: To investigate the effect of surface treatment on the biocompatibility of Ni-Ti shape memory alloy. METHODS: Ni-Ti shape memory alloy was randomly divided into two groups: experiment group’s materials were surface-treated by cathode electrodeposition; the others not treated were belonged to blank group. We observed the changes of material surface by scanning electron microscope and evaluated the hemolysis rate and kinetic coagulation time. The bone marrow stromal stem cells were cultured with two groups’ materials in vitro, and then we evaluated the MTT value of the two groups. RESULTS AND CONCLUSION: After surface treatment of Ti-Ni shape memory alloy, there were many nano-size particles on its surface that formatting Ti-O. In the experimental group, the hemolysis rate was decreased and the kinetic coagulation time was prolonged. The MTT values in the experimental group were significantly higher than those in the blank group at the 2nd, 4th and 6th days after co-culture (P < 0.05). Ni-Ti shape memory alloy treated by cathode electrodeposition has the better biocompatibility and biological security.