| 人脐血间充质干细胞的体外成骨 |
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| 引用本文： | 王浩,未东兴.人脐血间充质干细胞的体外成骨[J].中国组织工程研究与临床康复,2011,15(40):7446-7449. |
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| 作者姓名： | 王浩 未东兴 |
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| 作者单位： | 1. 辽宁医学院第五临床医院骨科,辽宁省锦州市121000;锦州市中心医院骨科,辽宁省锦州市121000
2. 锦州市中心医院骨科,辽宁省锦州市,121000 |
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| 摘 要： | 背景:用免疫细胞阴性筛选法可以排除造血干细胞对于间充质干细胞生长的影响,较快的获得具有表达间充质干细胞的细胞群。目的:探讨人脐静脉血间充质干细胞向成骨样细胞分化的能力。方法:用免疫磁珠阳性筛选法分离脐血间充质干细胞后进行培养。取P2代细胞行成骨诱导分化。结果与结论:人脐血间充质干细胞贴壁生长,长梭形,3周长满瓶底,传代后细胞增殖迅速。细胞表型为高表达CD44+,CD29+和CD166+。在成骨诱导后碱性磷酸酶染色阳性,Von-Kossa染色提示钙化结节形成。提示脐血间充质干细胞能够在体外分化为成骨样细胞。
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| 关 键 词： | 脐血 间充质干细胞 诱导分化 种子细胞 细胞表型 |
Osteogenic differentiation of mesenchymal stem cells from human cord blood in vitro |
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| Wang Hao,Wei Dong-xing.Osteogenic differentiation of mesenchymal stem cells from human cord blood in vitro[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(40):7446-7449. |
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| Authors: | Wang Hao Wei Dong-xing |
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| Institution: | Wang Hao1,2,Wei Dong-xing2 1Department of Orthopedic Surgery,the Fifth Clinical Hospital of Liaoning Medical University,Jinzhou 121000,Liaoning Province,China,2Department of Orthopedic Surgery,Jinzhou Central Hospital |
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| Abstract: | BACKGROUND: Negative screening for immune cells can remove the effect of hematopoietic stem cells on the growth of mesenchymal stem cells, with rapid access to obtain cell populations to express mesenchymal stem cells. OBJECTIVE: To study the osteogenic ability of mesenchymal stem cells from human cord blood. METHODS: The mesenchymal stem cells were isolated by immunomagnetic beads from cord blood. P2 cells were to study the ability of osteogenesis. RESULTS AND CONCLUSION: The human cord blood mesenchymal s... |
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