| 人脐血单个核细胞多次静脉移植家兔急性心肌梗死后的胶原重构 |
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| 引用本文： | 李文斌,余国龙,袁春菊,杨天伦.人脐血单个核细胞多次静脉移植家兔急性心肌梗死后的胶原重构[J].中国组织工程研究与临床康复,2011,15(45):8413-8417. |
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| 作者姓名： | 李文斌 余国龙 袁春菊 杨天伦 |
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| 作者单位： | 1. 中南大学湘雅医院心内科,湖南省长沙市,410008;湘潭市中心医院心内科,湖南省湘潭市,411100
2. 中南大学湘雅医院心内科,湖南省长沙市,410008 |
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| 摘 要： | 背景:以往干细胞移植治疗心肌梗死的研究,均为单次经静脉或冠脉注射移植。目的:进一步验证人脐血单个核细胞多次静脉移植对家兔急性心肌梗死胶原重构及心功能影响。方法:取家兔45只结扎冠脉左前降支制备急性心肌梗死模型,随机分为3组,①多次移植组:造模后7,9,11,13d经耳缘静脉注入BrdU标记人脐血单个核细胞生理盐水。②单次移植组:造模后7d注入BrdU标记人脐血单个核细胞生理盐水,9,11,13d注入生理盐水。③心梗对照组:仅注入生理盐水。另取家兔5只为假手术组。结果与结论:与对照组相比,两移植组心功能左室短轴缩短率、左室射血分数明显升高(P<0.05);且多次移植组改善效果优于单次移植组(P<0.05);免疫组织化学显示两移植组造模后2,4周心梗周边区均存在BrdU阳性细胞,但多次移植组BrdU阳性细胞计数多于单次移植组;改良Masson’s染色显示与假手术组比较,对照组梗死区及非梗死区胶原密度显著增加,梗死区域胶原纤维部分融合,排列较紊乱,心肌基本组织结构破坏;与对照组比较,两移植组造模后2,4周梗死周边区域心肌细胞间胶原含量、胶原纤维明显减少,胶原纤维排列较为有序;与单次细胞移植组比较,多次细胞移植组进一步改善。提示多次静脉移植脐血单个核细胞改善急性心肌梗死心功能、阻抑心肌胶原纤维重构疗效优于单次静脉移植。
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| 关 键 词： | 人脐血单个核细胞 静脉移植 急性心肌梗死 胶原纤维 重构 |
Effects of multi-intravenous transplantation of human cord blood mononuclear cells on the remodeling of cardiac collagen after myocardial infarction in rabbits |
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| Li Wen-bin,Yu Guo-long,Yuan Chun-ju,Yang Tian-lun.Effects of multi-intravenous transplantation of human cord blood mononuclear cells on the remodeling of cardiac collagen after myocardial infarction in rabbits[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(45):8413-8417. |
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| Authors: | Li Wen-bin Yu Guo-long Yuan Chun-ju Yang Tian-lun |
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| Institution: | Li Wen-bin1,2,Yu Guo-long1,Yuan Chun-ju1,Yang Tian-lun1 1Department of Cardiology,Xiangya Hospital of Central South University,Changsha 410008,Hunan Province,China,2Department of Cardiology,Xiangtan Central Hospital,Xiangtan 411100 |
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| Abstract: | BACKGROUND:In previous studies,the treatment of myocardial infarction by using stem cell transplantation was done via single intravenous or coronary injection transplantation.OBJECTIVE:To investigate the effects of multi-intravenous transplantation of human cord blood mononuclear cells(HCBMCs) on the remodeling of cardiac collagen and heart function after myocardial infarction in rabbits.METHODS:Forty-five Chinese rabbits underwent ligation of the left anterior coronary artery for the preparation of myocard... |
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