| 仓鼠深Ⅱ度烧伤创面愈合过程中糜蛋白酶的表达 |
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| 引用本文： | 董祥林,耿中利,高伟成,赵阳,秦涛,乔星,马娟,马少林. 仓鼠深Ⅱ度烧伤创面愈合过程中糜蛋白酶的表达[J]. 中国组织工程研究与临床康复, 2011, 15(46). DOI: 10.3969/j.issn.1673-8225.2011.46.031 |
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| 作者姓名： | 董祥林 耿中利 高伟成 赵阳 秦涛 乔星 马娟 马少林 |
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| 作者单位： | 1. 新疆医科大学第一附属医院烧伤整形科,新疆维吾尔自治区乌鲁木齐市,830054
2. 新疆医科大学附属肿瘤医院乳腺头颈外科,新疆维吾尔自治区乌鲁木齐市,830011 |
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| 基金项目： | 新疆医科大学第一附属医院青年科学基金 |
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| 摘 要： | 背景:肥大细胞糜蛋白酶在烧伤创面表达的研究较少.目的:观察肥大细胞糜蛋白酶是否存在于烫伤创面以及深Ⅱ度烫伤后不同时间点的表达变化.方法:应用75 ℃的水接触仓鼠背部皮肤12 s制备深Ⅱ度烫伤创面(病理组织学证实).分别于烫伤前及烫伤后1,3,7,14 d 取烫伤组织进行试验.结果与结论:实时定量RT-PCR 及放射免疫检测结果显示烫伤后创面组织中糜蛋白酶mRNA 表达量和活性均有所增高,在烫伤后第3 天最高.表明肥大细胞糜蛋白酶在烧伤创面有所表达,并可能参与了烧伤损伤愈合过程.
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| 关 键 词： | 深Ⅱ度烧伤 糜蛋白酶 肥大细胞 创面 愈合 仓鼠 |
Chymase expression in deep partial-thickness burn wounds after burn injury in hamsters |
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| Dong Xiang-lin,Geng Zhong-li,Gao Wei-cheng,Zhao Yang,Qin Tao,Qiao Xing,Ma Juan,Ma Shao-lin. Chymase expression in deep partial-thickness burn wounds after burn injury in hamsters[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(46). DOI: 10.3969/j.issn.1673-8225.2011.46.031 |
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| Authors: | Dong Xiang-lin Geng Zhong-li Gao Wei-cheng Zhao Yang Qin Tao Qiao Xing Ma Juan Ma Shao-lin |
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| Affiliation: | Dong Xiang-lin1,Geng Zhong-li2,Gao Wei-cheng1,Zhao Yang1,Qin Tao1,Qiao Xing1,Ma Juan1,Ma Shao-lin1 1Department of Burns and Plastic Surgery,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,Xinjiang Uygur Autonomous Region,China,2Department of Breast,Head and Neck Surgical Oncology,Affiliated Tumour Hospital of Xinjiang Medical University,Urumqi 830011 |
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| Abstract: | BACKGROUND: Little is known about mast cell chymase in burn injury in hamsters.OBJECTIVE: To investigate whether mast cell chymase is involved in burn injury and the change of chymase expression in burnwound.METHODS: Deep partial-thickness scald in hamsters was induced with contact time of 12 seconds by 20 mL 75 ℃ water in a50 mL syringe. Chymase mRNA level and chymase activity were analyzed before burn and at 1, 3, 7, 14 days after burn in deepsecond-degree scald wound. RESULTS AND CONCLUSION: Quantitative real-time RT-PCR and radioimmunoassay results showed that chymase geneexpression and chymase activity in burn wound were significantly increased after burn and were highest at 3 days after burn.Expression of mast cell chymase would be involved in burn injury and changed at different stages after burn in hamsters. |
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