| 三维培养条件下胚胎鼠唾液腺上皮细胞的自组装 |
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| 引用本文： | 王军胜,黄桂林,张霓霓,姚礼,唐岳鹏. 三维培养条件下胚胎鼠唾液腺上皮细胞的自组装[J]. 中国组织工程研究与临床康复, 2011, 15(40). DOI: 10.3969/j.issn.1673-8225.2011.40.032 |
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| 作者姓名： | 王军胜 黄桂林 张霓霓 姚礼 唐岳鹏 |
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| 作者单位： | 1. 西安医学院附属医院口腔科,陕西省西安市,710077
2. 遵义医学院附属口腔医院口腔颌面外科,贵州省遵义市,563003 |
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| 基金项目： | 贵州省优秀科技教育人才省长资金,贵州省教育厅基金 |
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| 摘 要： | 背景:对唾液腺胚胎细胞在体外自组装及形态发生过程的理解,将有利于在体外构建唾液腺器官样组织的研究.目的:探讨三维培养条件下SD大鼠胚胎鼠下颌下腺上皮细胞的自组装机制.方法:将胚胎期15 d鼠的下颌下腺上皮细胞分为实验组(加入抗E-cadherin及抗β-catenin)和对照组(常规培养),在Matrigel中三维培养.结果与结论:获得的胚胎鼠下颌下腺上皮细胞均表达CK19.实验组细胞未见分枝状及腺泡样结构出现;E-cadherin 和β-catenin荧光表达弱.对照组细胞在12 d出现上述结构;胞浆中E-cadherin、β-catenin荧光表达强表达.MOD值为实验组<对照组(P < 0.01).提示E-cadherin,β-catenin在细胞的自组装过程中有重要作用.
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| 关 键 词： | 下颌下腺上皮细胞 胚胎鼠 三维培养 自组装 E-cadherin β-catenin |
Self-organization and branching morphogenesis of primary salivary epithelial cells under three-dimensional culture |
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| Wang Jun-sheng,Huang Gui-lin,Zhang Ni-ni,Yao Li,Tang Yue-peng. Self-organization and branching morphogenesis of primary salivary epithelial cells under three-dimensional culture[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(40). DOI: 10.3969/j.issn.1673-8225.2011.40.032 |
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| Authors: | Wang Jun-sheng Huang Gui-lin Zhang Ni-ni Yao Li Tang Yue-peng |
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| Affiliation: | Wang Jun-sheng1,Huang Gui-lin2,Zhang Ni-ni2,Yao Li2,Tang Yue-peng2 1Department of Stomatology,Affiliated Hospital of Xi'an Medical College,Xi'an 710077,Shaanxi Province,China,2Department of Oral and Maxillofical Surgery,Stomatological Hospital,Zunyi Medical College,Zunyi 563003,Guizhou Province |
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| Abstract: | BACKGROUND: To understand self-assembly and branching of primary salivary epithelial cells will provide insights into reconstructing artifical salivary gland orgnoid. OBJECTIVE: To investigate the machanism of self-organization and branching mophogenesis of primary submandibular epithelial cells under three-dimensional condition. METHODS: The embryonic day 15 (E 15) mouse submandibular gland epithelium were inoculated in matrigel for 3-dimantional culture with/without (Group A/Group B) anti-E-cadherin and a... |
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