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| 骨髓间充质干细胞静脉移植治疗大鼠脊髓损伤 | |
| 引用本文： | 于长申,管雅琳,王世民,徐小林,王新平,阎晓玲,张雪青. 骨髓间充质干细胞静脉移植治疗大鼠脊髓损伤[J]. 中国组织工程研究与临床康复, 2011, 15(45): 8463-8468. DOI: 10.3969/j.issn.1673-8225.2011.45.023 |
| 作者姓名： | 于长申 管雅琳 王世民 徐小林 王新平 阎晓玲 张雪青 |
| 作者单位： | 1. 天津医科大学研究生院,天津市,300070 2. 天津市环湖医院神经内科,天津市,300060 3. 天津市环湖医院病理科,天津市,300060 4. 天津市环湖医院神经电生理科,天津市,300060 |
| 基金项目： | 天津市卫生局科技基金支持项目(05KY17),课题名称:骨髓间充质干细胞移植治疗大鼠脊髓损伤的实验研究~~ |
| 摘 要： | 背景:干细胞移植可以重建中枢神经系统的结构和功能,近年来引起了广泛的关注。目的:尾静脉移植骨髓间充质干细胞观察其对大鼠脊髓损伤修复的影响并探讨其机制。方法:密度梯度离心法结合贴壁筛选法分离骨髓间充质干细胞。将成年雌性Wistar大鼠以动脉瘤夹钳夹法制备大鼠脊髓损伤模型后随机分为细胞移植组和对照组,分别干预。结果与结论:细胞移植组大鼠BBB后肢功能评分恢复优于对照组(P<0.05)。自损伤后第30天开始,细胞移植组大鼠运动诱发电位潜伏期及体感诱发电位P1波潜伏期的恢复均优于对照组(P<0.05),并持续至实验结束。细胞移植组大鼠脊髓内在损伤中心及头、尾端距离脊髓损伤中心1cm处均可见阳性细胞。说明尾静脉注射移植骨髓间充质干细胞可以促进脊髓损伤大鼠神经功能恢复,其机制可能与移植细胞迁移到损伤部位,分化为神经元样和神经胶质细胞样细胞,并分泌或促进宿主分泌神经营养因子有关。 |
| 关 键 词： | 脊髓损伤 骨髓间充质干细胞 静脉移植 细胞移植 神经功能 |
Intravenous transplantation of bone marrow mesenchymal stem cells in the treatment of spinal cord injury in rats |
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| Yu Chang-shen,Guan Ya-lin,Wang Shi-min,Xu Xiao-lin,Wang Xin-ping,Yan Xiao-ling,Zhang Xue-qing. Intravenous transplantation of bone marrow mesenchymal stem cells in the treatment of spinal cord injury in rats[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(45): 8463-8468. DOI: 10.3969/j.issn.1673-8225.2011.45.023 | |
| Authors: | Yu Chang-shen Guan Ya-lin Wang Shi-min Xu Xiao-lin Wang Xin-ping Yan Xiao-ling Zhang Xue-qing |
| Affiliation: | Yu Chang-shen1,Guan Ya-lin2,Wang Shi-min2,Xu Xiao-lin2,Wang Xin-ping2,Yan Xiao-ling3,Zhang Xue-qing4 1Postgraduate College,Tianjin Medical University,Tianjin 300070,China,2Department of Neurology,3Department of Pathology,4Department of Neurophysiology,Huanhu Hospital of Tianjin,Tianjin 300060 |
| Abstract: | BACKGROUND:Transplantation of stem cells can rebuild the structure and function of injured central nervous system,and has attracted wide attention in recent years.OBJECTIVE:To explore the effects and mechanisms of bone marrow mesenchymal stem cells(BMSCs) transplanted through the vena caudalis on neurological recovery of rats with spinal cord injury.METHODS:BMSCs were separated with density gradient centrifugation and adherent screening methods.Forty adult female Wistar rats were used to establish the model... |
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