粉尘螨Der f2变应原的分离纯化及其特征

目的 用粉尘螨（Dermatophagoides farinae）代谢培养基浸液（Dff）分离纯化粉尘螨2类变应原（Der f2）。方法 Sephadex G-100层析、DEAE离子交换层析、PAGE等方法分离纯化Der f2，并对Der f2作SDS-PAGE测定和热稳定性测定。结果 从Dff分离纯化得到较纯Der f2，经SDS-PAGE测定其相对分子质量为14000，并经100℃加热15min后，仍保留50%～83.3%生物学活性。结论 从Dff中分离纯化的变应原，其物化性质符合Der f2特征。

Purification and Characterization of Der f2 Allergen in Dermatophagoides f arinae

Purpose To purify Der f2 allergen from the spent culture medium extract of Dermatophagoides farinae mite(Dff). Methods We purified Der f2 with the aid of Sephadex G-100 gel filtration, DEAE-ion exchange chromatography and polyacrylamide gel electrophoresis, detected the molecular weight of Der f2 using SDS-PAGE method,and analysed the thermostability of Der f2 by heating at 100℃ for 15 min. Results The pure Der f2 was obtained from Dff. The molecular weight of Der f2 was 14 000.The heated-treated Der f2 retained 50% ～83.3% of the activity of the native allergen. Conclusions The properties of purified allergen coincided with the characterization of Der f2.