Extracellular matrix from human umbilical cord-derived mesenchymal stem cells as a scaffold for peripheral nerve regeneration |
| |
Institution: | 1. Institute of 0rthopedics, Chinese PLA General Hospital, Beijing, China;2. Department of 0rthopedics, First Afifliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China;3. Department of 0rthopedics, Cangzhou Central Hospital, Cangzhou, Hebei Province, China |
| |
Abstract: | The extracellular matrix, which includes collagens, laminin, or fibronectin, plays an important role in peripheral nerve regeneration. Recently, a Schwann cell-derived extracellular matrix with classical biomaterial was used to mimic the neural niche. However, extensive clinical use of Schwann cells remains limited because of the limited origin, loss of an autologous nerve, and extended in vitro culture times. In the present study, human umbilical cord-derived mesenchymal stem cells (hUCMSCs), which are easily accessible and more proliferative than Schwann cells, were used to prepare an extracellular matrix. We identiifed the morphology and function of hUCMSCs and investi-gated their effect on peripheral nerve regeneration. Compared with a non-coated dish tissue culture, the hUCMSC-derived extracellular matrix enhanced Schwann cell proliferation, upregulated gene and protein expression levels of brain-derived neurotrophic factor, glial cell-derived neurotrophic factor, and vascular endothelial growth factor in Schwann cells, and enhanced neurite outgrowth from dorsal root ganglion neurons. These ifndings suggest that the hUCMSC-derived extracellular matrix promotes peripheral nerve repair and can be used as a basis for the rational design of engineered neural niches. |
| |
Keywords: | nerve regeneration peripheral nerve regeneration mesenchymal stem cells extracellular matrix Schwann cell dorsal root ganglion neuron niche neurotropic factors collagen laminin fibronectin neural regeneration |
本文献已被 CNKI 万方数据 等数据库收录! |
|