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白天挤粘法检测在校大学生蠕形螨感染效果分析
引用本文:张军,王伟,傅皓,宋辉,王耀辉,王国英. 白天挤粘法检测在校大学生蠕形螨感染效果分析[J]. 中国病原生物学杂志, 2012, 0(6): 454-455,485
作者姓名:张军  王伟  傅皓  宋辉  王耀辉  王国英
作者单位:河南大学医学院免疫学研究所;河南大学生命科学学院
基金项目:河南大学第十一批校级教学改革项目实验教学改革资助课题(No.101)
摘    要:目的分析白天挤粘法检出蠕形螨感染的情况。方法采用挤粘法对434名在校大学生进行蠕形螨检查,在显微成像系统下观察并记录拍照,同时调查受检者个人卫生习惯。结果 434名学生中158人感染蠕形螨,感染率为36.41%,男生感染率为26.79%,女生感染率为42.48%,女生显著高于男生(χ2=9.64,P<0.01);毛囊蠕形螨面部感染率(69.62%)高于皮脂蠕形螨(17.09%)(χ2=44.38,P<0.01);卵检出率19.60%,高于常规检查方法的卵检出率。检出的螨虫在透明胶纸上保存时间较长,20d左右蠕形螨的形态依然清晰可辨。结论白天挤粘法取材方便快速,操作简单,观察标本时间足够,适合临床检验和大样本人群普查。

关 键 词:人体蠕形螨  感染  大学生

Analysis of Demodex infection among college students using skin scrapings collected with cellophane tape during the daytime
ZHANG Jun,WANG Wei,FU Hao,SONG Hui,WANG Yao-hui,WANG Guo-ying. Analysis of Demodex infection among college students using skin scrapings collected with cellophane tape during the daytime[J]. Journal of Pathogen Biology, 2012, 0(6): 454-455,485
Authors:ZHANG Jun  WANG Wei  FU Hao  SONG Hui  WANG Yao-hui  WANG Guo-ying
Affiliation:1(1.Medical Collage,Henan University,Kaifeng,Henan475004,China;2.College of Life Sciences,Henan University)
Abstract:Objective To analyze the incidence of Demodex infection using skin scrapings collected with cellophane tape during the daytime.Methods Four hundred and thirty-four medical students were tested for Demodex mite infection using skin scraping and the cellophane tape method.Samples were observed and photographed with a microscopic imaging system.Health habits of the students were investigated via a questionnaire.Results The total rate of infection was 36.4 %.The infection rate for boys was 26.79% and that for girls was 42.48%.Boys had a higher infection rate than girls(χ2=9.64,P<0.01).The rate of infection with D.folliculorum(69.62%)was higher than that with D.brevis(17.09%)(χ2=44.38,P<0.01).The rate of ova detection was 19.6%.This is higher than the rate of ova detection by a routine examination.Mites on transparent adhesive tape can be preserved for a relatively long period of time,and Demodex mites are still evident after about 20 days.Conclusion Skin scrapings collected with cellophane tape during the daytime are a convenient and fast method of detecting Demodex.This method is easy to perform and specimens can be viewed for a sufficient amount of time.This method is suitable for clinical testing and surveys of large populations.
Keywords:Human Demodex  infection  college student
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