| 鱼藤卤虫致死活性成分的分离和结构鉴定 |
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| 引用本文： | 蒋诚,熊本强,邱细敏,刘胜姿,张偲,尹浩. 鱼藤卤虫致死活性成分的分离和结构鉴定[J]. 中药材, 2012, 0(5): 719-723 |
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| 作者姓名： | 蒋诚 熊本强 邱细敏 刘胜姿 张偲 尹浩 |
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| 作者单位： | 湖南师范大学医学院;中国科学院南海海洋研究所 |
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| 基金项目： | 湖南省教育厅重点项目(10A071);广东省自然科学基金(91510301000001) |
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| 摘 要： | 目的:研究鱼藤Derris trifoliata Lour.根和茎乙酸乙酯提取物的卤虫致死活性成分。方法:以卤虫2龄幼虫为试虫,采用活性跟踪指导,使用硅胶柱色谱、Sephadex LH-20和半制备液相色谱分离纯化,根据理化性质和光谱学数据进行结构鉴定。结果:分离鉴定了11个化合物,其中12a-羟基鱼藤酮、灰叶素、鱼藤酮和鱼藤素的卤虫致死活性LD50分别为0.365、0.236、0.060和0.734μg/mL。结论:活性测试表明,12a-羟基鱼藤酮、灰叶素、鱼藤酮和鱼藤素均具有显著的卤虫致死活性。
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| 关 键 词： | 鱼藤 卤虫致死活性 结构鉴定 |
Isolation and Identification of Brine Shrimp Lethal Activities from Derris trifoliata |
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| JIANG Cheng,XIONG Ben-qiang,QIU Xi-min,LIU Sheng-zi,ZHANG Si,YIN Hao. Isolation and Identification of Brine Shrimp Lethal Activities from Derris trifoliata[J]. Journal of Chinese medicinal materials, 2012, 0(5): 719-723 |
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| Authors: | JIANG Cheng XIONG Ben-qiang QIU Xi-min LIU Sheng-zi ZHANG Si YIN Hao |
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| Affiliation: | 1.Department of Pharmacy,Medical College,Hunan Normal University,Changsha 410006,China;2.Key Laboratory of Marine Bio-resourses Sustainable Utilization,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China.) |
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| Abstract: | Objective:To study the brine shrimp lethal activities of the roots and stems from Derris trifoliata.Methods:The biological activity of the athyl acetate extract was tested by the 2nd-instar of Artemia franciscana by the method of Silica gel column chromatography,Sephadex LH-20 column chromatography,Semipreparative HPLC,and their structures were elucidated by spectral analysis.Results:Eleven compounds were isolated from the athyl acetate extract,and LD50s of 12a-hydroxyrotenone,tephrosin,rotenone and deguelin were 0.365,0.236,0.060 and 0.734 μg/mL,respectively.Conclusion:The results of bioassay show that 12a-hydroxyrotenone,tephrosin,rotenone and deguelin have strong brine shrimp lethal activity. |
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| Keywords: | Derris trifoliata(Lour.) Shrimp lethal activity Structure identification |
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