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Molecular epidemiology of human herpesvirus 8 variants in Kaposi's sarcoma from Iranian patients |
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| Authors: | Jalilvand Somayeh Tornesello Maria Lina Buonaguro Franco M Buonaguro Luigi Naraghi Zahra Safaie Shoja Zabihollah Ziaee Abed-Ali Hamkar Rasool Shahmahmoodi Shohreh Nategh Rakhshandeh Mokhtari-Azad Talat |
| Affiliation: | a School of Public Health, Tehran University of Medical Sciences, Tehran, Iran b Molecular Biology and Viral Oncology and AIDS Reference Centre, National Cancer Institute “Fond. G. Pascale”, Naples, Italy c Razi Hospital, University of Medical Sciences, Tehran, Iran d Institute of Biochemistry and Biophysics, University of Tehran, Iran |
| Abstract: | Kaposi's sarcoma (KS) is a rare cancer in Iran and there is no epidemiological and molecular information about HHV-8 variants circulating among the Iranian population. In this study HHV-8 sequences have been analyzed in 43 cutaneous KS biopsies from Iranian patients mainly affected by classic KS. DNA samples were subjected to PCR amplification of HHV-8 ORF26, T0.7 and K1 followed by direct nucleotide sequencing and phylogenetic analysis. The analysis of ORF26 showed that 30 (69.8%) and 13 (30.2%) samples belonged to subtypes A/C and K, respectively. In general, the clustering of HHV-8 T0.7 variants paralleled that of ORF26. Genotyping of K1 sequences showed that the majority of samples (39 out of 41) fall into the large C clade with only 2 belonging to the A clade. In conclusion, HHV-8 variants identified among classic Iranian KS are largely related to Eurasian genotypes previously identified in KS from Mediterranean, Middle East, and East Asian regions. |
| Keywords: | Kaposi's sarcoma Human herpesvirus 8 ORF26 ORF K1 ORF T0.7 Iran |
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