Extraction of components from hydrocortisone mixtures using high pressure thin layer chromatography

Chromatographic conditions for the separation of five pairs of active compounds occuring in four ointment preparations and an aerosol one were elaborated. The ways of extraction of hydrocortisone esters (acetate and butyrate), chlorquinaldol, oxytetracycline base and oxytetracycline hydrochloride from the ointments as well as purification of the extracts prior to the HPLC analysis were described. The proposed analytical methods are for more specific and precise from those used until now in the home-made preparations quality control.