Rearrangement generated in double genes, NSP1 and NSP3, of viable progenies from a human rotavirus strain |
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Authors: | Kojima K Taniguchi K Kawagishi-Kobayashi M Matsuno S Urasawa S |
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Institution: | Department of Hygiene, Sapporo Medical University School of Medicine, Japan. kkojima@sapmed.ac.jp |
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Abstract: | We generated rotavirus clones with rearrangement in vitro by serial passages of a human rotavirus strain (IGV-80-3) at high multiplicity of infection and determined nucleotide sequences of the rearranged genes from two distinct rotavirus clones, each of which possesses two rearranged genes: a common rearranged NSP1 gene and NSP3 gene with slightly different migration in polyacrylamide gel electrophoresis. Sequence analysis showed that the rearranged NSP1 and NSP3 genes had similar gene structures: concatemerization in a head to tail orientation and partial duplication of the open reading frame following the termination codon. The rearranged NSP1 gene had a direct repeat, whereas in the rearranged NSP3 gene, no such pattern was found. |
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