白藜芦醇联合姜黄素对SMMC-7721肝癌细胞作用

目的:观察白藜芦醇联合姜黄素对体外人肝癌细胞SMMC-7721增殖和凋亡的影响及相关信号通路.方法:不同浓度白藜芦醇、姜黄素及两药联合干预SMMC-7721细胞,MTT法检测细胞增殖,流式细胞术检测细胞凋亡、Hoechst 33258染色检测细胞凋亡形态变化,比色法检测半胱氨酸天冬氨酸蛋白酶(caspase)-3,caspase-8,caspase-9酶活性,Western blot法检测半胱氨酸天冬氨酸蛋白酶切割底物(PARP).结果:与对照组相比,白藜芦醇、姜黄素单独或联合作用SMMC-7721细胞均可抑制SMMC-7721细胞增殖,两药联合后抑制作用更显著.白藜芦醇、姜黄素联合较单独用药可增强SMMC-7721细胞凋亡,呈现凋亡形态改变,白藜芦醇、姜黄素及联合组细胞凋亡率分别为( 17.39±1.41)％,(14.96±2.23)％,(25.36±2.68)％;同时提高SMMC-7721细胞caspase-3,caspase-8及caspase-9活性,促使PARP蛋白剪辑.结论:白藜芦醇、姜黄素联合使用可增强对人肝癌细胞SMMC-7721的抗癌作用,并可能与caspase-8,caspase-9/caspase-3/PA RP信号通路介导细胞凋亡相关.

Combination of Resveratrol and Curcumin Inhibits Proliferation and Induces Apoptosis in Human Hepatocarcinoma SMMC-7721 Cells

Objective: To observe the combinational effects of resveratrol and curcumin on cell proliferation, apoptosis and the possible mechanisms in human hepatocarcinoma SMMC-7721 cells in vitro. Method: SMMC-7721 cells were treated with resveratrol or curcumin or both. Cell proliferation was detected by MTT assay. Cell apoptosis was detected by flow cytometry, apoptotic morphology was visualized by hoechst 33258 staining. Caspase-3, caspase-8 and caspase-9 activities were detected by colorimetric assay, and cleaved poly(ADP-ribose) polymerase(PARP) was detected by Western blot. Result: Compared with the control, resveratrol and curcumin significantly inhibited the proliferation of SMMC-7721 cells. The combination of resveratrol and curcumin was found to be more effective in inhibiting growth (P<0.01), and inducing apoptosis in SMMC-7721 as indicated by apoptotic morphological change and PI/Annexin V-FITC staining. The apoptosis rate of resveratrol, curcumin and combination group was (17.39±1.41)%, (14.96±2.23)%, and (25.36±2.68)% respectively. In addition, caspase-3, caspase-8 and caspase-9 were significantly activated by combinational treatment of resveratrol and curcumin, accompanied by increased PARP cleavage, and compared with either agent alone. Conclusion: Combination of resveratrol and curcumin may elicit synergistic anti-cancer effects in human hepatocarcinoma SMMC-7721 cells, and associated with caspase-8, -9/caspase-3/PARP mediated apoptosis.