荞精补肾胶囊对小肠葡萄糖吸收的体外抑制作用

目的:观察荞精补肾胶囊(QJBS)体外抑制小肠葡萄糖吸收的作用.方法:SD大鼠禁食不禁水24 h,处死,制备小肠离体外翻模型,随机分为空白对照组、阿卡波糖对照组(0.25 g·L-1)和QJBS 1 ～ QJBS 5 (2.5,5.0,10.0,20.0,40.0 g·L-1).将肠囊分别放人等量的营养液中,加入麦芽糖及受试药物,在37℃水浴箱中孵育120 min,测定葡萄糖浓度并计算抑制率.结果:QJBS各组对离体小肠葡萄糖的吸收具有抑制作用,当药物质量浓度为20.0 g·L-1,抑制作用最明显,抑制率为(67.7±10.5)％,与阿卡波糖抑制作用相当.结论:QJBS能够抑制小肠对葡萄糖的吸收,其作用可能与抑制肠道α-糖苷酶类有关.

Inhibition of Qiaojing Bushen Capsule on Intestinal Glucose Absorption in vitro

Objective: To observe inhibition of Qiaojing Bushen capsules(QJBS) in vitro on glucose absorptionin small intestinal.Method: SD rats were fasted and gave water for 24 hours,then sacrificed to prepare small intestine in vitro everted model.Rats were divided into the blank control group,and acarbose tablets group 0.25 g·L-1 and QJBS group: QJBS 1-QJBS 5(2.5,5.0,10.0,20.0,40.0 g·L-1).The sugar substrate and tested drugs were added into gut sac,which were incubated for 120 min at 37 ℃ to determin glucose concentrations,and the inhibition rate was calculated.Result:Each group of QJBS can inhibit small intestinal absorption of glucose,when the drug concentration was 20 g·L-1,inhibitory effect was the most obvious.The inhibition rat was(67.7±10.5)%,which was equal to acarbose.Conclusion: QJBS can inhibit intestinal glucose absorption,the effect may be related to inhibition of intestinal α-glucosidase.