体重对糖尿病肾病大鼠尿蛋白检测的影响分析

目的:探讨体重对实验性糖尿病肾病大鼠尿蛋白检测的影响.方法:清洁级健康雄性SD大鼠72只,随机分为糖尿病组(DM组,n=45)和对照组(NC组,n=27),糖尿病组大鼠一次性腹腔注射STZ 60 mg· kg-1.两组大鼠分别在第4,8,12,16周留取24 h尿液,用放免法检测尿白蛋白,监测体重(BW),同时检测肾脏功能和形态.结果:尿白蛋白/肌酐(UACR)在两组间无差异于；尿白蛋白(UTP),尿白蛋白排泄率(UAER),UTP/BW,UAER/BW,UACR/BW在4个时间点DM组高于NC组(P＜0.05)；5种尿蛋白不同评价指标与肾脏功能形态指标无相关性.结论:体重对糖尿病大鼠UACR的指标评价影响最明显.5种尿白蛋白的评价指标在糖尿病肾病早期不能动态完全反映肾脏病理损害变化.

Influence of Body Weight on Urine Albumin Levels Detected in Experimental Diabetic Nephropathy Rats

Objective: To compare the influence of body weight in diagnostic importance for experimental diabetic nephropathy rats among three urinary protein screening methods. Method: Seventy-two SPF male Sprague-dawley rats were randomLy divided into control group (NC, n=27) and diabetic group(DM, n=45), which were induced with streptozotocin once intraperitoneal injection. At 4,8,12, 16 w after injection,the 24 h urine albumin levels were measured by immunoturbidime-try.The body weight and general renal function index were monitored and the tissue sections of renal were examined with lightmicroscopy. Result: The levels of UTP,UAER,UTP/BW,UAER/BW and UACR/BW were higher in diabetic rats than that of the normal group(P<0.05),but there was no difference in UACR between the two groups.There was no correlation between the levels of the five different urinary albumin screening methods and renal-related indicators in diabetic rats. Conclusion: These data indicate that the body weight only affect the diagnostic importance of UACR and the five different urinary albumin screening methods could not be a perfect marker in diagnosis of diabetic early-stage nephropathy and indicator of the developing process of glomerulosclerosis.