新疆天然植物黑加仑对食管癌细胞增殖、凋亡的影响

目的:观察新疆天然植物黑加仑水提物对食管癌细胞的影响及其作用机制。方法:采用四甲基偶氮唑蓝(MTT)法测定不同浓度(10-1、10-2、10-3g/ml)黑加仑水提物作用不同时间(12、24、36h)对人食管癌细胞株Eca109的细胞存活量的影响,以大鼠正常肝细胞(BRL)为正常对照细胞株;用Bradford法测定肿瘤细胞蛋白质合成的变化;采用流式细胞技术(FCM)观察黑加仑作用后肿瘤细胞周期变化及凋亡情况;通过倒置显微镜观察细胞的形态变化。结果:黑加仑水提物10-1g/ml组对人食管癌细胞株Eca109作用24h后,癌细胞的生长和蛋白合成均有明显抑制作用(P<0.05),对正常肝细胞的存活量及蛋白合成均无影响;流式细胞仪检测二倍体峰前出现凋亡峰,细胞凋亡率为49.6%;并且癌细胞呈现典型的凋亡细胞形态。结论:黑加仑水提物可通过诱导细胞凋亡而抑制人食管癌细胞株Eca109细胞的生长。

EFFECT OF BLACK CURRANT ON PROLIFERATION AND APOPTOSIS OF HUMAN ESOPHAGEAL CANCER CELL LINE IN VITRO

Objective: To investigate the effect of black currant extract on human esophageal squamous cancer cell Eca109 line to reveal its probable mechanism. Methods: The survival cells and protein synthesis of tumor cell lines treated with 10-1, 10 -2,10-3 g/ml of the water extracts of black currant for 24 h were determined by MTT and Bradford assays, and the cell-DNA ploidy distribution and apoptotic rate were measured by flow cytometry (FCM) and morphological observation. Results: There was significant inhibition of the cell survival and protein synthesis of cancer cells at dose of 10-1 g/ml of the extracts. In the normal rat liver cells, the cell survival was not affected. An apoptosis peak appeared before diploid peak in FCM and apoptotic rate was 49.6%, and morphological change of apoptosis was observed. Conclusion: Black currant could significantly inhibit growth of human esophageal squamous cancer cell Eca109 line through inducing apoptosis.