电针对炎症痛大鼠痛阈及中缝大核、中缝背核和导水管周围灰质c-fos表达的影响

越来越多的研究均表明 ,不同类型的伤害性刺激可诱发ｃ ｆｏｓ在大鼠、小鼠、猫和豚鼠的脊髓和不同脑区中表达。本实验观察了大鼠在角叉菜胶致炎后 ,在痛觉调制密切相关的中缝大核、中缝背核和导水管周围灰质ｃ ｆｏｓ表达的变化 ,及电针镇痛作用对ｃ ｆｏｓ表达的影响。实验分成正常对照组、角叉菜胶致炎组和电针 角叉菜胶致炎组。大鼠脚掌注射角叉菜胶 (2 % ,0 .1ｍＬ)造成大鼠炎症痛模型 ,测定大鼠的痛阈变化。电针组动物 ,在角叉菜胶致炎前电针大鼠“足三里”和“昆仑”穴(疏密波 ,2Ｈｚ和 6 0Ｈｚ交替 ,强度 1～ 3ｍＡ) 30ｍｉｎ。大…

The Effects of Acupuncture on c-fos Expression in the Brain of Carrageenan Inflammation Rats

The immediate early gene c fos is rapidly and transiently expressed in neurons in response to stimulation. The aim of the present study is to observe the effec ts of acupuncture on c fos expression of periaqueductal gray(PAG), nucleus raph e magnus (NRM)and dorsal raphe nucleus(DRN) in carrageenan inflammation rats.Rats were divided into control, carrageenan inflammation and acupuncture groups. Except control group, each rat was received a unilateral injection of carrageen an (Sigma, 2 mg/100 μL per paw) into the hind paw. The results showed that the nociceptive threshold of control and inflammation rats were 13.34±2.11 sec and 1.74±0.22 sec indicating that the hyperalgesia was induced by carrageena n. The number of the labeled Fos LI neurons in PAG, NRM and DRN was significant ly increased (18.90±1.79 to 44.25±2.91, P<0.001, 2.57±0.84 to 9.60 ±1.88, P<0.05 and 15.33±1.59 to 31.39±3.55,P<0.001 ,respe ctively). Electroacupuncture (EA) unilateral "Zusanli"(ST 36) and "Kunlun"(BL 60) acupoints(2 and 60 Hz alternately,1～ 3 mA, 30 min) significantly elevated the nociceptive threshold of the carrageena n injected paws(P<0.05). At the same time, in the EA treated rats the number of labeled Fos LI neurons was significantly decreased in PAG and DRN (44 .25±2.91 to 17.86±1.63,P<0.01, 31.39±3.55 to 18.27±2.02,P< 0.05), but not in NRM(9.60±1.88 to 8.83±2.21,P>0.05). The re sults indicate that the EA supressed the carrageenan inflammation induced Fos ex pression in PAG and DRN, but not in NRM in rats.