Preferential utilisation of deoxycytidine by undifferentiated (peanut positive) tonsillar lymphocytes |
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| Authors: | J Taljanidisz T Spasokukotskaja M Sasvári-Székely F Antoni M Staub |
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| Institution: | 1. Faculty of Pharmacy, Department of Biochemistry, Gazi University, Ankara, Turkey;2. Department of Basic Oncology, Istanbul University, Oncology Institute, Istanbul, Turkey;3. Department of Surgery, Istanbul University, Institute of Oncology, Istanbul, Turkey;4. Faculty of Pharmacy, Department of Toxicology, Gazi University, Ankara, Turkey;1. Department of Respiratory Medicine, First Affiliated Hospital of Xi''an JiaoTong University, Xi''an, Shaanxi 710061, People''s Republic of China;2. Wuhan University of Science and Technology, Wuhan, Hubei 430081, People''s Republic of China;3. Division of Allergy and Clinical Immunology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA |
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| Abstract: | Peanut agglutinin (PNA), a D-galactose specific lectin, agglutinated 10-18% of lymphocytes isolated from tonsils of 3- to 6-yr-old children. PNA+ cells were found to be mainly B lymphocytes showing a 9.6 times higher specific activity of DNA polymerase alpha compared to the PNA- cells. The specific activity of deoxycytidine kinase as well as the incorporation of 5-3H]deoxycytidine were also much higher in PNA+ cell fraction than in PNA- fraction (7.7-fold and 6-fold, respectively). On the other hand, thymidine kinase activity and 5-3H]deoxythymidine incorporation were only 3.6 and 3.9 times higher, respectively. The data presented here show a high degree of DNA synthesis and preferential utilisation of 5-3H]deoxycytidine for DNA synthesis in this undifferentiated B lymphocyte fraction. |
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