尖锐湿疣组织HPV基因芯片分型及型别分析

目的研究尖锐湿疣(CA)组织中人乳头瘤病毒(HPV)感染的病毒亚型分布,CA患者中高危型HPV感染的情况。方法采用基因芯片检测和分型方法,对武汉市302例CA患者的皮损组织进行HPV检测及分型。结果302份CA组织标本中HPV阳性286例,检出率为94.7%,单一型别阳性率为73.8%,多重型别阳性率为20.7%,低危型别(HPV6,11,42,43,44)阳性率为80.5%,高危型别(HPV16,18,31,33,35,39,45,51,52,53,56,58,59,66,68,73,83,MM4)阳性率为14.2%,对照组阳性率为16.7%。结论本地区尖锐湿疣以单一型别及低危型别HPV感染为主,高危型HPV感染阳性率为14.2%。

Typeing Analysis and Classification of HPV with Gene Chips in Condyloma Acuminata

Objective To study genotyping of HPV-DNA in condyloma Acuminata . The infection of the high-risk HPV in Condyloma Acuminata.Methods 302 cases lesions were excised from patients with condyloma acuminata were detected classification of HPV-DNA with gene chips.Results The results showed that the positive rates of the HPV in 286 HPV positive samples were detected from 302 clinic samples,the total positive rate was 94.7%,the positive rate of single types of HPV was 73.8%(223/302),the positive rate of multiple types of HPV was 20.7%, the positive rate of low-risk HPV(6,11,42,43,44)was 80.5%,the positive rate of high-risk HPV(16,18,31,33,35,39,45,51,52,53,56,58,59,66,68,73, 83,MM4) was 14.2%(43/302), the positive rate of GP-HPV in normal group was 16.7%(18/108).Conclusion HPV6 and HPV11 infection was the main cause in condylona acuminata in Wuhan Area, the positive rates of the high-risk HPV infection was 14.2%.