滋补脾阴方药对脾阴虚糖尿病大鼠下丘脑胰岛素信号的调控作用

目的：通过观察胰岛素信号通路中关键分子的变化，进一步探讨脾阴虚糖尿病认知功能障碍的发病机制及滋补脾阴方药的作用机制，以期为糖尿病认知功能障碍的治疗提供新思路和新线索。方法：将大鼠随机分为空白对照组（Cont），糖尿病组（DM），脾阴虚组（pi），脾阴虚糖尿病组（piDM），滋补脾阴方药治疗组（ZBPYR）5 组。采用Western blotting 的方法观察下丘脑中胰岛素受体底物1（IRS-1）丝氨酸磷酸化水平及蛋白激酶C（PKC/Akt）丝氨酸磷酸化水平，以判定胰岛素信号传导是否出现障碍。结果：DM 组、pi组、piDM 组p-IRS-1ser 表达较Cont 组增加（P<0.05），DM 组、piDM 组p-Akt 表达减弱（P<0.05）。ZBPYR 组p-IRS-1ser 较DM 组、piDM 组减弱（P<0.05），p-Akt 表达较DM 组、piDM 组增加（P<0.05）。结论：DM 组、pi组、piDM 组大鼠下丘脑中胰岛素信号未正常向下传导，可能发生了胰岛素信号转导障碍。ZBPYR 具有纠正胰岛素信号转导障碍的作用。

Regulation of Insulin Signaling in the Hypothalamus of Spleen Yin Deficiency Diabetes Rats Treated with Zibu Piyin Recipe

This study was aimed to observe changes of key molecular in insulin signaling pathway in the hypothalamus of rats to explore the mechanism of spleen yin deficiency diabetes-associated cognitive decline (DACD) and Zibu Piyin Recipe (ZBPYR) in order to provide new ideas and new clues for treatment of DACD. Rats were randomly divided into five groups, which were the control (Cont) group, diabetes (DM) group, spleen yin deficiency (pi) group,spleen yin deficiency diabetes (piDM) group and the ZBPYR group. Insulin receptor substrate 1 (IRS-1) serine phosphorylation levels and protein kinase B (PKB/Akt) serine phosphorylation levels which were involved in the insulin signaling were observed by western blotting in the hypothalamus to determine whether there were insulin signaling obstacles in the hypothalamus of rats. The results showed that the expression of p-IRS-1ser in the DM group, pi group and piDM group was increased compared with the Cont group (P < 0.05); while the p-Akt expression of the DM group and piDM group was decreased (P < 0.05). The expression of p-IRS-1ser in the ZBPYR group decreased compared with the DM group and piDM group (P < 0.05); while the level of p-Akt increased compared with the DM group and piDM group (P < 0.05). It was concluded that insulin signaling was not transduced normally in the hypothalamus of the DM group, pi group and piDM group. Insulin resistance may occur in the hypothalamus. And ZBPYR can correct insulin signaling transduction disorder.