质谱成像技术及其在药学领域的应用

质谱成像是一种在组织、细胞或亚细胞水平研究分子或离子种类及分布的新型分析技术，无需复杂前处理即可实现样本表面多种物质的原位定性、定量分析，绘制出它们的空间分布图。质谱成像技术在药学领域正崭露出其独特的魅力和优势。本文介绍了该技术的原理、样品制备、离子化技术，综述了其在药物代谢研究、药效毒理评价、药物蛋白质组学探讨、靶向药物筛选及固体制剂评价等方向的应用。

Mass spectrometry imaging and its application in pharmaceutical sciences

Mass spectrometry imaging(MSI)is a new analytical technique to study the composition and distribution of molecules or ions at tissue, cellular or subcellular level. It can detect analytes qualitatively and quantitatively on sample surface in situ without complex pre-treatment, mapping their spatial distribution. MSI technique is now emerging with its advantages in pharmaceutical science. Principle, sample preparation and ionization techniques concerning MSI are introduced in this review. Its application in drug metabolism study, pharmacodynamic and toxicological evaluation, pharmacoproteomics exploration, targeted drug screening and solid preparations investigation are also reviewed.