宽叶缬草对动脉平滑肌细胞收缩及生长的影响

目的 :观察宽叶缬草 (VOL)对培养的血管平滑肌细胞 (VSMC)收缩及生长的影响。方法 :取 4～ 6周wistar大鼠胸主动脉中层平滑肌细胞进行原代及传代培养。观察VOL和L -NAME对VSMC收缩的影响 ,以及血管紧张素Ⅱ (AngⅡ )和不同浓度VOL时VSMC的3H -TdR及3H -Leucine参入变化。结果 :VOL可显著抑制AngⅡ引起的VSMC收缩 ,且此作用不受L -NAME影响。VOL呈剂量依赖性抑制VSMC的3H -TdR和3H -Leucine的参入。结论 :VOL有显著抑制AngⅡ引起的VSMC收缩和生长的作用

Valeriana Officinalis Var.Latifolia Miq Regulates Vascular Smooth Muscle Cell Contraction and Growth

Objective To investigate the effects of valeriana officinalis var .Latifolia miq (VOL) on the contraction and growth of cultuted rat vascular smooth muscle cell(VSMC). Method Aortic medial smooth muscle cells of wistar rat were cultured and passaged. The VSMCs were stimulated to contract by angiotentin Ⅱ(Ang Ⅱ 10 -3 mmol/L), and the contraction were measured. 3H-TdR and 3H-Leucine incorporation of VSMCs were performed at different time points after the addition of drugs. Results VOL could significantly inhibit the Ang Ⅱ-stimulated contraction of VSMCs, L-NAMEcould not affect VOL's ability to inhibit contraction. VOLcould significantly inhibit the incorporation of 3H-TdR and 3H-Leucine into VSMCs in does-dependent manner. Conclusion VOL could significantly suppress the Ang Ⅱ-stimulated contraction and growth of cultured rat VSMCs.