大鼠心冠状动脉系统神经肽Y年龄变化的免疫组织化学研究

运用免疫组化ＡＢＣ法对不同月龄ＳＤ大鼠心脏冠状动脉及其分支含神经肽Ｙ神经纤维的分布及其衰老变化作了详细观察．结果表明：左、右冠状动脉及其各级分支具有丰富的神经肽Ｙ能神经分布．在冠状动脉主干及其较大分支上，神经纤维较稠密，主要呈环状或网状分布，在血管围周形成较多束、丛、网，并伸入血管壁内形成壁内的神经网络分布．部分神经纤维的游离末梢有穿过血管内膜伸向腔面的迹象．在细小的冠状动脉分支上，神经纤维密度减低，主要沿血管长袖是纵向分布．两侧冠状动脉系统神经肽Ｙ能纤维分布形式及密度没有差别．老龄组动物，左、右冠状动脉及其分支的神经肽Ｙ能纤维密度趋于下降，纤维变细，呈断续状，膨体数量减少，免疫染色变浅、此外，在心内发现了神经肽Ｙ能神经节．

IMMUNOHISTOCHEMICAL RESEARCH ON NEUROPEPTIDE Y IN CORONARY ARTERY SYSTEM OF RAT HEART

In order to investigate the distribution and aging change of NPY-containing nerve fibers in the coronary artery systpm of heart, 2～4, 10～12 and 18～20 month-old SD rats (n=10, n=10, n=8, respectively) were used by means of immunohistochemical method (avidin -biotin -peroxidase complex technique, ABC). The results are as follows: In right a nd left coronary artery and their branches there were abundant NPY-containing nerve fibers. The fiber was with many varicosities. The fibers may be in single or in bundle, plexus and network. In trunks and branches of coronary artery, the density of NPY fibers was the highest, and the fibers were mainly distributed in circle, surrounded the vessels. In mid-sized branches, the fibers were in network. In small branches, the number of fibers was attenuated and the fibers went along the longitudinal axis of the vessels. It was important that some NPY fibers passed through the wall of coronary vessels and their endings tended to extend into the lumen. The densities of NPY fibers of right and left coronary artery systems showed no differencer. In addition, some NPY positive nervous ganglia have been observed in a few of specimens. They were placed in the posterior wall of atria and made from NPY-containing cell bodies with aging, especially in old rats, the NPY fibers innervated the blood vessels tended to attenuate. The density went down. The numbers of nerve bundle, plexus and network were reduced. The fiber itself got thinner and the stain got paler. The number of varicosities was decreased. These changes might mean that the NPY nerve innervation of the coronary vessels were weakened in old animal.