神经生长因子促进MIA PaCa-2人胰腺癌细胞的增殖

目的：观察神经生长因子-β(nerve growth factorbeta, NGFβ)对人胰腺癌MIA PaCa-2细胞增殖及细胞周期的影响。方法:体外培养MIA PaCa-2细胞，单独或联合给予不同浓度的NGF-β和K252a (NGF-β受体TrKA的抑制剂)，应用克隆平板实验、MTT法和流式细胞术检测NGF-β和K252a对MIA PaCa-2细胞克隆形成率、增殖及细胞周期的影响。结果：NGF-β显著促进MIA PaCa-2细胞的克隆形成（P<0.05），NGF-β使MIA PaCa2细胞增殖能力明显增强（P<0.01），K252a抑制MIA PaCa-2细胞增殖（P<0.05），NGF-β与K252a联合作用对MIA PaCa-2细胞的增殖能力无明显影响。NGF-β作用使MIA PaCa2细胞周期阻滞于S期，K252a作用使其周期阻滞于G0/G1期，两者联合作用使MIA PaCa-2细胞周期阻滞于S期。结论：NGF-β具有促进胰腺癌MIA PaCa-2细胞增殖的作用。

Nerve growth factor promotes proliferation of human pancreatic cancer cell line MIA PaCa-2

Objective: To investigate the effect of nerve growth factor-β(NGF-β) on the proliferation and cell cycle of human pancreatic cancer MIA PaCa-2 cells. Methods: MIA PaCa-2 cells were treated with different concentrations of NGF-β and K252a (inhibitor of NGF-β receptor TrKA) alone or in combination. Clone forming rate, proliferation, and cell cycle of MIA PaCa-2 cells treated with different strategies were examined by clone formation assay, MTT, and flow cytometry, respectively. Results: NGF-β significantly increased the clone formation and proliferation of MIA PaCa-2 cells (P<0.05, P<0.01). K252a significantly inhibited the proliferation of MIA PaCa-2 cells (P<0.05), while NGF-β combined with K252a had no significant effect on the proliferation of MIA PaCa-2 cells. NGF-β arrested MIA PaCa-2 cell cycle in S phase, K252a arrested cell cycle in G_0/ G_1 phase, and NGF-β combined with K252a arrested cell cycle in S phase. Conclusion: NGF-β can enhance the proliferation of pancreatic carcinoma MIA PaCa-2 cells.