| 人甲状腺球蛋白的提取和纯化 |
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| 引用本文： | 叶静,汤特.人甲状腺球蛋白的提取和纯化[J].天津医科大学学报,2000,6(3):295-297. |
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| 作者姓名： | 叶静 汤特 |
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| 作者单位： | 叶静（天津医科大学病理生理教研室）;汤特（天津医科大学病理生理教研室）;张天庚（天津医科大学病理生理教研室）;陆凤仙（天津医科大学病理生理教研室） |
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| 摘 要： | 目的：提取高纯度的甲状腺球蛋白（Ｔｇ），为制备Ｔｇ抗血清及Ｔｇ单克隆抗体，建立Ｔｇ的检验方法及ＴｇＡｂ的测定方法做准备。方法：将手术切除的新鲜人甲状腺组织，加入生理盐水匀浆、过滤，把滤液浓缩，经二次ＳｅｐｈａｄｅｘＧ－２００柱层析，再经ＤＥＡＥ纤维素离子交换层析。结果：获得较高纯度的人Ｔｇ，经ＳＤＳＰＡＧＥ鉴定，显示为１条区带，表明纯度较高，经免疫电泳鉴定，提纯的Ｔｇ有较好的免疫活性。结论：这种提
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| 关 键 词： | 甲状腺球蛋白 甲状腺 葡聚糖凝胶层析 |
| 文章编号： | 1006-8147(2000)03-295-03 |
| 修稿时间： | 2000年1月19日 |
Isolation and purification of human thyroglobulin |
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| YE Jing,TANG Te,ZHANG Tian-geng,LU Feng-xian.Isolation and purification of human thyroglobulin[J].Journal of Tianjin Medical University,2000,6(3):295-297. |
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| Authors: | YE Jing TANG Te ZHANG Tian-geng LU Feng-xian |
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| Abstract: | Objective: To establish the immunoassay of thyroglobulin (Tg) and anti-Tg antibody (TgAb), with use of Tg antiserum and Tg monoclonal antibody by immunization of highly purified Tg. Methods: A Surgical specimen of human thyroid tissue was homogenized in normal saline, filtered and then concentrated. The homogenate was purified on a colamn of Sephadex G-200 twice and further passed through a DEAE cellulose column. Results:Highly purified Tg was obtained. Single band from purified Tg was found in SDS-PAGE, indicating that the purity of Tg was high. The immune activity of purified Tg kept well by immune electrophoresis identification. Conclusion: The method for preparing Tg can be used for large production of Tg antigen with respect of performing easily and isolating completly. |
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| Keywords: | thyroglobulin thyroid gland sephadex chromatography lon exchange chromatography |
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