| 血管紧张素Ⅱ对培养乳鼠非心肌细胞DNA及蛋白合成的影响 |
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| 引用本文： | 王洪新,陶亮,饶曼人. 血管紧张素Ⅱ对培养乳鼠非心肌细胞DNA及蛋白合成的影响[J]. 中国药理学通报, 1996, 0(5) |
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| 作者姓名： | 王洪新 陶亮 饶曼人 |
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| 作者单位： | 南京医科大学心血管药理研究室，锦州医学院药理教研室 |
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| 摘 要： | ＡｕｇⅡ作为一种独立于血液动力学及神经体液因子，对哺乳动物心脏细胞的直接作用尚未完全清楚。本实验中，我们观察了ＡｕｇⅡ对培养心脏的非心肌细胞的作用，结果表明：在培养的非心肌细胞，ＡｕｇⅡ引起蛋白合成、ＤＮＡ合成及细胞数目的明显增加，但不影响细胞的体积。ＡｕｇⅡ在非心肌细胞诱导的ＤＮＡ合成受ＡｕｇⅡ受体拮抗剂［Ｓａｒ１，Ｖａｌ５，Ａｌａ８］－ＡｕｇⅡ完全抑制、这些结果提示：（１）ＡｕｇⅡ诱导心脏非心肌细胞的增殖；（２）ＡｎｇⅡ的上述作用通过ＡＴ１受体亚型调控。
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| 关 键 词： | 培养，非心肌细胞，血管紧张素Ⅱ，心肌肥厚 |
Effects of angiotensin Ⅱ on DNA and protein synthesis in cultured cardiac nonmyocytes of neonatal rats |
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| Wang Hong-Xin,Tao Liang, Rao Man-Ren. Effects of angiotensin Ⅱ on DNA and protein synthesis in cultured cardiac nonmyocytes of neonatal rats[J]. Chinese Pharmacological Bulletin, 1996, 0(5) |
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| Authors: | Wang Hong-Xin Tao Liang Rao Man-Ren |
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| Abstract: | Increasing evidence suggests that angiotensin Ⅱ(AugⅡ)may act as a growth factor for the heart.However,direct effects of Aug Ⅱon cardiac nonmyocytes-independent of secondary hemodynamic and neurohumoral effects have not been well characterized.In this experiment, we observed effects of Aug Ⅱon cultured cardiac nonmyocytes.The results showed that Ang Ⅱ1,10,100,1000nmol·L-1 induced increases in protein synthesis,DNA synthesis and cell number without changing cell volume in nonmyocytes.The Aug Ⅱ-induced DNA synthesis in nonmyocytes was fully blocked by[Sar1,Val5,Ala8]-Aug Ⅱ which suggested that:(1) Aug Ⅱcaused hyperplasla of cardiac nonmyocytes(2)The effects of AugⅡ examined were mediated primarily by the AT1 receptor subtype. |
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| Keywords: | culture nonmyocytes Angiotensin Ⅱ cardiac hypertrophy |
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