黑曲霉转化牛蒡子水提液中牛蒡子苷的研究

目的采用黑曲霉Aspergillus niger ZJUT302产生的β-葡萄糖苷酶水解牛蒡子水提液中的牛蒡子苷,提高牛蒡子苷元的量。方法 A.niger ZJUT302在培养基成分为稻草粉50 g/L、麦麸15 g/L、大麦粉15 g/L、(NH4)2SO4 10 g/L、KH2PO4 0.5 g/L、MgSO4.7H2O 0.5 g/L,温度30℃,初始pH 5.0,添加0.1%聚乙二醇5000,摇床转速150 r/min的优化反应条件下转化牛蒡子水提液7 d。结果牛蒡子苷在初始浓度0.06 mmol/L时,转化率可达92.3%。结论本方法是一种有效的牛蒡子生物炮制技术。

Transformation of arctiin in water extract of Arctii Fructus with Aspergillus nigger

Objective To explore the optimal separation of the araloside A from Aralia chinensis by selecting appropriate macroporous resins and to systematically study the factors which affect the separation. Methods Static and dynamic adsoption-desorption methods were adopted and evaluated for separating efficiency by measuring the concentration of araloside A in A. chinensis with HPLC. Results Macroporous resin AB-8 had the best separating efficiency when the content in A. chinensis liquid was 0.1 g/mL equivalent to raw materia1. The volume of drug is 6 BV (resin bed volume) with the adsorption-power 2.5 BV/h and the volume of 50% ethanol as eluant 4 BV with desorption-power 2 BV/h. After the treatment of AB-8 resin, the purity of araloside A could reach 30% and total araloside to 80%. Conclusion This method is simple and feasible with good effect of separation, which can meet the industrial requirements.