| 豚鼠膀胱组织ICCs细胞间隙连接蛋白Connexin 43表达意义 |
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| 引用本文： | 鄢俊安,卢根生,李龙坤,宋波.豚鼠膀胱组织ICCs细胞间隙连接蛋白Connexin 43表达意义[J].第三军医大学学报,2008,30(7):561-563. |
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| 作者姓名： | 鄢俊安 卢根生 李龙坤 宋波 |
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| 作者单位： | 第三军医大学西南医院全军泌尿外科研究所,重庆,400038 |
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| 摘 要： | 目的 探讨体外培养豚鼠膀胱组织ICCs细胞(interstitial cells of Cajal,ICCs)间隙连接蛋白Connexin 43的表达.方法 胶原酶消化法进行豚鼠膀胱组织ICCs细胞的原代培养, 对照为原代培养膀胱平滑肌细胞,免疫荧光法进行两种细胞的特征性蛋白c-kit及平滑肌肌动蛋白(smooth muscle actin,SMA)染色观察,免疫荧光法及Western blot法进行培养细胞Connexin43的检测.结果 豚鼠膀胱ICCs细胞特征性蛋白c-kit染色阳性,SMA染色阴性.豚鼠膀胱ICCs细胞间隙连接蛋白Connexin 43表达水平较高,显著高于膀胱平滑肌细胞.结论 豚鼠离体膀胱ICCs细胞间隙连接蛋白Connexin43高表达可能是其信号传递的重要物质基础.
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| 关 键 词： | Cajal间质细胞 间隙连接蛋白 膀胱 |
| 文章编号： | 1000-5404(2008)07-0561-03 |
| 修稿时间： | 2007年12月5日 |
Connexin 43 expression in cultured interstitial cells of Cajal from guinea pigs |
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| YAN Jun-an,LU Gen-sheng,LI Long-kun,SONG Be.Connexin 43 expression in cultured interstitial cells of Cajal from guinea pigs[J].Acta Academiae Medicinae Militaris Tertiae,2008,30(7):561-563. |
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| Authors: | YAN Jun-an LU Gen-sheng LI Long-kun SONG Be |
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| Institution: | Urinary Surgery Research Institute;Southwest Hospital;Third Military Medical University;Chongqing 400038;China |
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| Abstract: | Objective To explore the expression of connexin 43 in the interstitial cells of Cajal (ICCs) from guinea pig bladder in vitro. Methods Bladder ICCs were primarily cultured from guinea pigs by collagenase digestion method. Primarily cultured bladder smooth muscle cells (SMCs) from guinea pig were taken as control. The expressions of c-kit and smooth muscle actin (SMA) were detected by immunofluorescent method. Immumofluorescent method and Western blotting were used to detect the expression of Connexin 43 in ... |
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| Keywords: | interstitial cells of Cajal gap junction protein bladder |
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