| 离子交换层析分离纯化重组人血清白蛋白 |
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| 引用本文： | 朱家文,武斌,陈葵,罗凌丽,周武,李水龙. 离子交换层析分离纯化重组人血清白蛋白[J]. 医学教育探索, 2002, 0(4): 341-345350 |
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| 作者姓名： | 朱家文 武斌 陈葵 罗凌丽 周武 李水龙 |
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| 作者单位： | [1]华东理工大学化学工程研究所，上海200237 [2]湖南岳阳中湘康神药业集团有限公司，湖南414000 |
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| 基金项目： | 国家自然科学基金资助项目 ( 2 9976 0 14 ) |
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| 摘 要： | 通过层析实验，比较了几种阴离子交换剂在不同温度、pH值、离子强度、进样流速、进样浓度下对人血清白蛋白的吸队与脱附性能，研究了它们对人血清白蛋白和卵清蛋白的分离能力，并考察了桩层析过程中吸附载体对重组人血清白蛋白和杂蛋白的分离选择性，实验结果表明：版号为DEAE Sepharose FF的载体不仅对重组人血清白蛋白具有良好的分离度，而且有较高的回收率。
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| 关 键 词： | 分离纯化 重组人血清白蛋白 离子交换层析 阴离子交换剂 穿透曲线 分离度 基因工程 分离能力 |
Separation and Purification of Recombinant Human Serum Albumin by Ion Exchange Chromatography |
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| ZHU Jia wen,WU Bin,CHEN Kui,LUO Ling li,ZHOU Wu,LI Shui long. Separation and Purification of Recombinant Human Serum Albumin by Ion Exchange Chromatography[J]. Researches in Medical Education, 2002, 0(4): 341-345350 |
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| Authors: | ZHU Jia wen WU Bin CHEN Kui LUO Ling li ZHOU Wu LI Shui long |
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| Abstract: | Adsorption and elution characteristics of human serum albumin (HSA) and recombinant HSA under different operation conditions, such as pH, ionic strength of buffer solution, flow rate and concentration of feed were investigated in fixed bed chromatography with three kinds of anion exchange gel beads as supports. Separation of HSA and ovalbumin in chromatography was studied. Selectivity between recombinant human serum albumin and hybrid proteins by anion exchange chromatography were discussed. It was experimentally shown that high resolution and recovery of recombinant HSA were obtained in fixed bed chromatography with DEAE Sepharose FF as support. |
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| Keywords: | human serum albumin adsorption ion exchange chromatography anion exchange gel beads breakthrough curve rate of resolution |
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