Cytotoxicity of citrinin in cultured kidney epithelial cell systems

Cytotoxicity of citrinin, a fungal metabolite and a common food contaminant, was evaluated in an established cell line, Madin-Darby bovine kidney (MDBK) cells and primary fetal bovine kidney (PFBK) cells. Citrinin is a known nephrotoxicant but produced a low order of cytotoxicity in cultured renal cells. A dose-dependent cytotoxic effect was observed at millimolar concentrations of the toxin. MDBK cells were more sensitive than the PFBK cells. The primary effect of this chemical was on the adherence of MDBK cells to the culture dish. Microscopic evaluation of morphologic changes indicated that cells elongated, flattened, swelled, and became rounded. The appropriateness of toxicity evaluation in culture systems in vitro is considered.