p21 Expression in colorectal carcinomas: a study on 103 cases with analysis of p53 gene mutation/expression and clinic-pathological correlations |
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Authors: | S Girlando P Slomp O Caffo M Amichetti R Togni G Dvornik L Tomio E Galligioni P Dalla Palma M Barbareschi |
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Institution: | (1) Department of Pathology, S. Chiara Hospital, I-38100 Trento, Italy e-mail: barbamat@tin.it Tel.: +39-0461-903202, Fax: +39-0461-903389, IT;(2) Department of Clinical Oncology, S. Chiara Hospital, Trento, Italy, IT;(3) Department of Radiotherapy, S. Chiara Hospital, Trento, Italy, IT |
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Abstract: | The WAF1/CIP1 gene product, p21, an inhibitor of cyclin-dependent kinases, is a critical downstream effector in the p53 pathway.
The expression of p21 in human neoplasms is heterogeneous, and may be related to p53 functional status. We evaluated p21 immunoreactivity
in 103 colorectal carcinomas (CC) in relation to the p53 gene and protein alterations and clinico-pathologic parameters. High
p21 expression (more than 10% reactive cells) was seen in 39% of cases. p21 staining was heterogeneous and often detected
in clusters of tumour cells; in some tumours p21 staining was more pronounced in superficial areas. No relation was seen between
p21 immunoreactivity and site of the tumours (right vs left), TNM stage and grade. p21 expression was related to p53 status
as evaluated with IHC or with SSCP analyses, low p21 expression usually being associated with p53 protein overexpression (P=0.048) and p53 gene alteration (P=0.005). The strongest associations were seen when the combined p53/p21 immunophenotype was compared with p53 gene alterations
(P=0.0002). These data support the hypothesis that p21 expression in CC is mainly related to p53 functional status, suggesting
that p21 expression could be an interesting adjunct in the evaluation of the functional status of the p53 pathway in CC.
Received: 22 February 1999 / Accepted: 21 June 1999 |
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Keywords: | p21 p53 Colon Immunohistochemistry Human |
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