自由基清除剂依达拉奉对脓毒性休克家兔的治疗作用

目的：观察依达拉奉(ED)对脓毒性休克家兔血气、血生化指标及动物存活率的影响。方法：动物脓毒性休克后1 h用3个剂量ED(1．6,0．8,0．4 mg·kg~(-1))治疗,测定4个时相点动脉血气及酸碱度,并测血乳酸(L)、乳酸脱氢酶(LDH)、过氧化氢酶(CAT)、丙二醛(MDA)、超氧化物歧化酶(SOD)、肌酐(Cr)及尿素氮(BUN)含量。观察24 h时动物存活情况。结果：家兔脓毒性休克4 h后出现代谢性酸中毒合并呼吸性酸中毒,L、LDH、BUN明显升高,SOD活性明显降低,动物死亡率为70％。不同剂量ED治疗后可改善血气、酸碱平衡紊乱,增强SOD和CAT活性,降低LD、MDA浓度及LDH活性。治疗后3个剂量组动物死亡率分别为0,0和33％,明显低于模型组(P<0．05)。结论：ED能增强脓毒性休克机体的抗氧化能力,降低脓毒性休克死亡率。

Therapeutical efficacy of free radical scavenger edaravone on septic shock rabbits

AIM:To investigate the effects of edaravone (ED),a novel free radical scavenger,on arte- rial blood gases,some plasma biochemical parameters,and survival rate in rabbits with septic shock.METH- ODS:After treatment with three different doses of ED (1.6,0.8,0.4 mg.kg~(-1)) to rabbits suffered septic shock, changes of arterial blood gases,blood acid-base scale,plasma levels of lactic acid,MDA,Cr and BUN;ac- tivities of LDH,CAT and SOD were determined in four different time phases.The mortality of animals in 24 h was observed.RESULTS:Four hours after septic shock,metabolic acidosis and respiratory alkalosis occurred,the blood levels of lactic acid,BUN and activity of LDH increased significantly but the activity of SOD reduced markedly;with death rate up to 70%.However,following the treatment with different doses of ED,the degrees of the derangement of blood gases and acid-base equilibrium were improved with enhancement of the plasma activities of SOD and CAT and decrease of the plasma levels of LD,MDA and activity of LDH.The death rate in treated groups was signifcantly lower than that of the model group (P＜0.05).CONCLUSION:ED can increase the protection that of organ and tissue against free radical-mediated injury,decrease the secondary damage of reactive oxygen species,and recover the metabolic derangement of septic shock.