卵巢早衰患者外周血CD4~+、Th1/Th2细胞分布研究

近年的研究提示，卵巢早衰与免疫紊乱有关。本研究用流式细胞仪分拣细胞，结合原位杂交的方法研究卵巢早衰患者外周血ＣＤ４＋Ｔ淋巴细胞Ｔｈ１／Ｔｈ２分布，旨在探讨卵巢早衰自身免疫损伤的机制。结果：卵巢早衰组Ｔｈ１细胞百分比（４９７６±９２２）明显高于对照组（２００６±７１０）（Ｐ＜０．００１）；Ｔｈ１／Ｔｈ２细胞比值（１１５±０１７）明显高于对照组（０６３±００９）（Ｐ＜０．００１）。提示Ｔｈ１，Ｔｈ２细胞调节失衡，可能与卵巢早衰的发生有关。

Distribution of CD4 lymphocyte Th1/Th2 subsets in peripheral blood of women with idiopathic premature ovarian failure

Objective: The pathogenic mechanism of premature ovarian failure (POF) remains unknown. Accumulated evidences indicate that abnormality in the immune system may be one of the major reasons and the imbalance of T helper type Ⅰ (Th1) and T helper type II (Th2) may play an important role in the process. Study of the distribution and functional state of Th1, Th2 may be helpful to elucidate the pathogenesis of POF. Methods: 13 patients with idiopathic POF and 7 women in reproductive age with normal cycle were enrolled in this study. The percentage of Th1 and Th2 cells from peripheral blood of patients and controls were studied using FITC labelled CD4 mAb to seperate CD4 + cell on FACs,in combination with in situ hybridization using Dig labelled IL2, IL6 cDNA probes. Results: Patients with POF had significantly higher percentage of Th1 cells as compared with the controls (49.76±9.22%, 20 06±7.10% respectively) ( P <0.001). The ratio of Th1/Th2 in patients with POF were significantly higher than that of controls (1.15±0.17, 0.63±0.09) ( P <0.001). Conclusions: The findings of this study suggest that patients with POF have increased Th1 cell activation, which may be related to the pathogenesis of POF.