大剂量高压氧治疗对脑梗死大鼠组织核因子κB的影响

【摘要】 目的 探讨大剂量高压氧治疗方案对永久性大脑中动脉闭塞大鼠的疗效以及高压氧对大鼠梗死部位周围脑组织核因子κB（nuclear factor-κB，NF-κB）影响。 方法 制备雄性Sprague-Dawley大鼠永久性大脑中动脉闭塞模型，随机分为高压氧组和对照组，每组32只，另设立伪手术组。使用Garcia神经行为学评分方法分别在术后24 h、5 d对大鼠进行神经行为学评分；应用2，3，5-三苯基氯化四氮唑（2，3，5-triphenyltetrazolium chlorid，TTC）方法对脑组织进行染色，观察24 h、5 d时大鼠脑组织梗死容积；取梗死部位周围脑组织，采用凝胶电泳迁移实验（electrophoretic mobility shift assay，EMSA）方法检测术后24 h、5 d时的NF-κB脱氧核糖核酸（deoxyribonucleic acid，DNA）结合活性。比较三组间上述指标的差异。 结果 术后24 h高压氧组神经行为学评分高于对照组［（13.33±1.53）vs（10.33±0.58），P<0.001］。术后24 h高压氧组梗死容积小于对照组［（139.73±33.59）vs（203.02±57.66），P=0.008］。术后5 d高压氧组梗死部位周围脑组织NF-κB活性低于对照组［（16.01±4.56）vs（50.28±9.13），P=0.035］。 结论 大剂量高压氧治疗方案在脑梗死后24 h内具有脑保护作用。大剂量高压氧可降低大鼠梗死部位周围脑组织NF-κB DNA结合活性。

Effects of Large Doses of Hyperbaric Oxygen on Nuclear Factor-κB Activity of Brain Tissue of Acute Permanent Middle Cerebral Artery Occlusion in Rats

Objective To investigate the effects of large doses of hyperbaric oxygen （HBO） on acute permanent middle cerebral artery occlusion （PMCAO） in rats and to examine whether hyperbaric oxygen treatment （HBOT） could influence NF-κB deoxyribonucleic acid （DNA） binding activity of peri-infarction region of brain tissue. Methods Sprague-Dawley rats were randomized to HBO group, control group, and sham group （n=32）. HBO group and control group underwent PMCAO surgery. HBOT began 5 h after surgery. HBOT was delivered at a dose of 2 absolute atmosphere （ATA） for 90 min. Control animals were exposed to air at ambient room pressure. Other rats underwent sham surgery. Neurological outcome was measured at 24 h and 5 d after surgery using Garcia score. 2, 3, 5-triphenyltetrazolium chlorid （TTC） pathological staining and Image J software were used to determine infarction volume at the point of 24 h, 5 d after surgery. NF-κB DNA binding activity of brain tissue was investigated at 24 h, 5 d after surgery using electrophoretic mobility shift assay （EMSA）. Results Garcia score of HBO group at the point of 24 h after PMCAO was significantly higher than that of control group （13.33±1.53] vs 10.33±0.58], P〈0.001）. Infarction volume of HBO group at the point of 24 h was smaller than that of control group （ 139.73±33.59] vs 203.02±57.66], P=0.008）. The gray level of NF-κB of HBO group at 5 d after PMCAO was lower than that of control group （ 16.01±4.56] vs 50.28±9.13], P=0.035）. Conclusion Large doses of hyperbaric oxygen could protect ischemic brain tissue at 24 h after surgery. Large doses of hyperbaric oxygen could decrease NF-κB DNA binding activity of peri-infarction region of brain tissue.