肝硬变和肝癌中细胞凋亡与增殖的关系

利用原位ＤＮＡ末端转移酶标记技术和ＳＰ免疫组化方法检测肝硬变和肝细胞癌（ＨＣＣ）组织中凋亡细胞和增殖细胞核抗原。结果显示：ＨＣＣ中凋亡细胞密度显著低于肝硬变，而增殖细胞密度明显高于肝硬变。肝硬变中凋亡细胞主要分布于假小叶周边形成“凋亡细胞带”；在癌组织中呈散在分布。提示：肝硬变“凋亡细胞带”的形成可能与肝血流改变有关；肝硬变癌变过程中可能存在细胞选择性增殖。

Study on relationship between apoptosis and proliferation of cells in liver cirrhosis and hepatocellular carcinoma]

Apoptosis and nuclear antigen of proliferating cells were detected by labelling technique of in situ terminal deoxynucleotide transferase and immunohistochemical method in liver cirrhosis and hepatocellular carcinoma(HCC). The density of apoptotic cells in HCC was significantly lower than that in cirrhosis, and the density of proliferating cells was much higher in HCC than that in cirrhosis. Apoptotic cells mainly distributed in the peripseudolobular region of cirrhosis and formed an apoptosis zone. But they scattered within the cancer tissue. The results suggest that the formation of apoptosis zone in cirrhosis may be related to the change of liver blood stream. Selective proliferation of cells may exist during carcinogenesis of liver cirrhosis.