骨关节病软骨细胞DNA氧化性损伤

从１０例临床手术中取得骨关节病标本，包括髋关节置换术的股骨头５例，膝关节骨关节病病灶清除的变性软骨４例，膝关节游离体１例，采用常规方法，坐标不本中提取ＤＮＡ，并通过ＨＰＬＣ－ＥＣ检测ＤＮＡ中的８－羟基－２‘－脱氧鸟苷。结果显示，ＤＮＡ琼脂糖凝胶电泳出现典型细胞程序化死亡特征的梯形改变，且ＤＮＡ氧化性损伤标志８－ＯＨｄＧ的含量增加，提示内源性ＤＮＡ氧化性损伤可能是骨关节病的分子病理机制。

Oxidative DNA damage of cartilage in osteoarthrosis]

In order to study molecular mechanism of osteoarthrosis, we used molecular techniques to detect DNA oxidative damage of cartilage. There were 10 samples from clinical operation including 5 cases of replacement of hip joint, 4 degenerative cartilage of knee osteoarthrosis, and 1 free body of knee joint. DNA from all samples was run out on an agarose gel and appeared classic nucleosomal ladders. (8-hydroxyl-2'-deoxyguanosine)/ml (8-OHdG) in DNA samples was measured by high-performance liquid chromatography with electrochemical detection (HPLC-EC). The results showed that the levels of 8-OHdG were increased to 0.11-0.26 ng.ml-1, average 0.177 ng.ml-1. Normal controls were lower than 0.05 ng.ml-1. The high 8-OHdG in DNA led to a misreading of affected templates and base-pair exchange, which could be a sensitive indicator about oxidative DNA damage, which was produced by free radicals or other DNA damage agents. The result suggests that oxidative DNA damage may be the molecular mechanism of osteoarthrosis.