| 用PCR检测消化道巨细胞病毒感染 |
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| 引用本文： | 范昌华 施凯. 用PCR检测消化道巨细胞病毒感染[J]. 湖南医科大学学报, 1998, 23(3): 229-231 |
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| 作者姓名： | 范昌华 施凯 |
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| 摘 要： | 应用多聚酶链反应（ＰＣＲ）检测５１例消化道疾病患者活检或手术标本的人类巨细胞病毒（ＨＣＭＶ）－ＤＮＡ，阳性率９．８０％；采用间接免疫荧光法随机检测其中４１例患者血清ＨＣＭＶ－ＩｇＭ。结果显示：消化道组织巨细胞病毒感染数低于血清ＨＣＭＶ－ＩｇＭ阳性数（Ｐ〈０．０１）；血清ＨＣＭＶ－ＩｇＭ检测中，恶性肿瘤和结肠炎患者ＨＣＭＶ感染均较对照组为高（Ｐ〈０．０１和Ｐ〈０．０５）。提示ＰＣＲ是消化道巨细胞病毒
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| 关 键 词： | 巨细胞病毒 消化系疾病 聚合酶链反应 |
Detection of human cytomegalovirus by PCR in patients with gastrointestinal disease] |
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| C Fan,K Shi,S Chen,Z Xia. Detection of human cytomegalovirus by PCR in patients with gastrointestinal disease][J]. Bulletin of Hunan Medical University, 1998, 23(3): 229-231 |
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| Authors: | C Fan K Shi S Chen Z Xia |
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| Affiliation: | Department of Microbiology, Hunan Medical University, Changsha. |
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| Abstract: | Human cytomegalovirus (HCMV) genomes was detected by PCR in 51 patients' biopsy or operation specimens with gastrointestinal disease. The positive rate was 9.80%. At the same time, 41 patients' serum HCMV-IgM was detected by indirect immunofluorescence assay (IFA); the positive rate was 46.34%. Patients with cancer and colitis exhibited a higher HCMV infection rate than those without cancer and colitis. The results suggest that PCR is sensitive and specific in detection of gastrointestinal HCMV infection, and the examination of biopsy or surgical specimen is superior than that of IFA of serum HMCV-IgG. |
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