从再生障碍性贫血患者检出阵发性睡眠性血红蛋白尿症异常细胞的观察

目的：探讨在再生障碍性贫血（再障）患者中检出阵发性睡眠性血红蛋白尿症（ＰＮＨ）异常细胞的发生率。方法：用荧光标记的抗ＣＤ５９抗体，以流式细胞仪检测缺失ＣＤ５９的异常细胞。结果：１０名正常人骨髓单个核细胞及外周血红细胞和中性粒细胞的ＣＤ５９标记率均＞９５％。２３例再障患者中约２／３标记率正常，另外１／３患者的标记率有不同程度的减低。结论：该法是从再障患者检出少量ＰＮＨ异常细胞的良好手段，可用以早期发现再障－阵发性睡眠性血红蛋白尿症综合征。

Detection of CD 59 - blood cells in patients with aplastic anemia

Objective:To detect paroxysmal nocturnal hemoglobinuria (PNH) clone in aplastic anemia (AA) patients. Methods: Flow cytometric technique along with antibodies against CD 59 was used to estimate the amount of GPI P deficient cells, characteristic of PNH abnormalities. Results: Among 23 cases of AA studied, 13 possessed no excessive percentage of CD 59 - cells in peripheral PMNs and RBCs. Eight of these 13 cases underwent bone marrow examination, and no increase of CD 59 - mononuclear cell (MNC) was found either. Three other AA patients had slightly increased amount (>5%) of CD 59 - cells in both peripheral blood and bone marrow. In seven cases, percentages of CD 59 - cells were normal in peripheral RBCs and PMNs but increased in bone marrow MNCs. Conclusion: Measurement of CD 59 - cells in periperal blood and bone marrow by flow cytometry may serve as the best way of detecting PNH clone in AA, and thus could be used for the early diagnosis of AA PNH syndrome.